Autologously transplanted dermal fibroblasts improved diabetic wound in rat model
| Autor: | Leyla Tapul, Aslı Erdoğan, Hasan Serdar Mutlu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Cell type Histology medicine.medical_treatment Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Dermis Diabetes mellitus medicine Animals Wound Healing integumentary system business.industry Growth factor Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine Fibroblasts medicine.disease Streptozotocin Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis business Wound healing medicine.drug |
| Zdroj: | Acta Histochemica. 122:151552 |
| ISSN: | 0065-1281 |
| Popis: | Healing of diabetic wounds are delayed due to late initiation and prolongation of the inflammatory phase, and inadequate growth factor synthesis, which may lead to chronic ulcers that may cause limb amputation, besides making the patients vulnerable to infections. In recent years, it has been extensively discussed whether different cell types transplanted to diabetic wound models accelerate wound healing. In this study, the effect of dermis-derived cells on Streptozotocin (STZ) induced experimental diabetic Sprague-Dawley rats were investigated. Animals were divided into 3 groups. First group was control, second group included diabetic animals with wounds. In the third group, firstly, skin specimens were obtained from animal’s back, and then primary explant culture was performed. STZ induced experimental diabetes was applied to these animals and then wound was opened. The cells grown in primary culture were transplanted autologously. In all three groups, the samples taken from the wound areas on the 5th and 15th days of the wound were examined at the level of histochemical and immunohistochemical and electron microscopy. In the study, it was observed that the decreasing α-SMA and KGF (FGF-7) expression in the early period especially in the case of experimental diabetes increased as a result of cell transplantation, and in the sections belonging to the experimental diabetic group, a large number of inflammatory cells in the wound area were removed from the environment. In the cell transplanted group, the collagen fiber bundles as if in the control group. As a result, healthy cells of dermis can act as mesenchymal stem cells under certain conditions and have a positive effect on diabetic wound healing. |
| Databáze: | OpenAIRE |
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