A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures

Autor:	Kees R. Libbenga, F. van Iren, Wolfgang Kreis, T. J. M. Blom
Rok vydání:	1991
Předmět:	Chromatography Fresh weight Mineralogy Plant Science General Medicine Sedimentation Biology Plant cell Suspension culture law.invention Laboratory flask Erlenmeyer flask Cell culture law Agronomy and Crop Science Quantitative analysis (chemistry)
Zdroj:	Plant cell reports. 11(3)
ISSN:	0721-7714
Popis:	A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this height, appeared highly correlated with the fresh weight of cells. This result was found with eight cell lines in two Laboratories. The method proved to be very suitable to allow routinely measurement of FW without the destruction of cells, from many samples, in a short time, during each phase of the growth cycle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1110cc50c8327f68b1005bdcddeea79d https://pubmed.ncbi.nlm.nih.gov/24213548 Zobrazit plný text záznamu Full text from SpringerLink