Development of IgG Mediated Antibody Dependent Cell-mediated Cytotoxicity (ADCC) in the Serum and Genital Mucosa of HIV Seroconverters
| Autor: | Linda L. Baum, Mariana Mata, Kathryn Anastos, Helen G. Durkin, Audrey L. French, Fareeha Mahmood, Marek Nowicki, Ruth M. Greenblatt, Mariam Aziz, Chenglong Liu, Elizabeth T. Golub |
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| Rok vydání: | 2015 |
| Předmět: |
Immunology
Human immunodeficiency virus (HIV) Acute infection chemical and pharmacologic phenomena Dermatology medicine.disease_cause Article Virology medicine Women Sex organ Antibody dependent cellular cytotoxicity Seroconversion Cervix Antibody-dependent cell-mediated cytotoxicity business.industry HIV virus diseases 3. Good health Seroconverters Infectious Diseases medicine.anatomical_structure Genital tract Vagina business |
| Zdroj: | Journal of AIDS & clinical research |
| ISSN: | 2155-6113 |
| DOI: | 10.4172/2155-6113.1000479 |
| Popis: | Background: We measured antibody-dependent cell mediated cytotoxicity (ADCC) activity in serum and genital fluids of heterosexually exposed women during HIV seroconversion. Methods: Plasma and cervico-vaginal lavage (CVL) fluid from 11 seroconverters (SC) were analyzed biannually from one year pre- to 6 year post-seroconversion using a 51Cr-release assay to measure HIV-1 gp120 specific ADCC. Results: No SC had significant HIV specific CVL ADCC activity before seroconversion or until 1.5 yr after seroconversion. One individual had a %Specific Release (SR) of 25.4 at 2 years, 26.7 at 3 years and 21.0 at 4 years after seroconversion in CVL. Another sample had 4.7% SR at 2 years, 5.3 at 3 years, 10.9 at 4 years, and 8.4 at 5 years after seroconversion in CVL. A third had no activity until 17% SR 5 years after seroconversion in CVL. A fourth showed activity of 36.5% SR at 6.5 years after seroconversion. Seven women had no ADCC activity in their CVL. Paired serum samples showed HIV specific ADCC activity prior to the appearance of CVL ADCC activity. Conclusions: HIV specific ADCC activity in CVL rose 2 years after seroconversion; ADCC was present in the serum prior to this time. These data suggest that genital tract ADCC activity is not present until well after acute infection. |
| Databáze: | OpenAIRE |
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