Risk Estimation of HNA-3 Incompatibility and Alloimmunization in Thai Populations
| Autor: | Kamphon Intharanut, Oytip Nathalang, Kanokpol Siriphanthong, Nipapan Leetrakool, Siriporn Nathalang |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Isoantigens
Blood transfusion Genotyping Techniques medicine.medical_treatment lcsh:Medicine Blood Donors Lung injury Thais Polymorphism Single Nucleotide Asian People Gene Frequency Isoantibodies Risk Factors Blood plasma Genotype Medicine Humans lcsh:Science Genotyping Whole blood Multidisciplinary Membrane Glycoproteins biology business.industry lcsh:R Membrane Transport Proteins biology.organism_classification Thailand Genotype frequency Blood Group Incompatibility Immunology lcsh:Q Female business Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 1, p e0116905 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included. HNA-3 genotyping was performed using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The observed frequencies of the HNA-3a/3a, HNA-3a/3b, and HNA-3b/3b genotypes were 0.528, 0.380, and 0.092 in central Thais and 0.600, 0.350, and 0.050 in northern Thais, respectively. The frequencies were used to estimate HNA-3 incompatibility and risk of HNA-3a alloimmunization. The HNA-3 incompatibility in central Thais (33.28%) was higher than northern Thais (28.75%), corresponding to a significantly higher probability of HNA-3a alloimmunization (P |
| Databáze: | OpenAIRE |
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