A Photoacoustic Probe for the Imaging of Tumor Apoptosis by Caspase‐Mediated Macrocyclization and Self‐Assembly
| Autor: | Jianhui Weng, Deju Ye, Jinbo Li, Quli Fan, Xiaoming Hu, Yuqi Wang, Yan Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Macrocyclic Compounds
medicine.medical_treatment Photoacoustic imaging in biomedicine Caspase 3 Apoptosis 010402 general chemistry Cleavage (embryo) 01 natural sciences Catalysis Photoacoustic Techniques medicine Distribution (pharmacology) Humans Caspase Chemotherapy biology Molecular Structure 010405 organic chemistry Chemistry Optical Imaging General Chemistry General Medicine 0104 chemical sciences Molecular Imaging Cyclization Caspases Molecular Probes biology.protein Cancer research Imaging Signal Glioblastoma |
| Zdroj: | Angewandte Chemie. 131:4940-4944 |
| ISSN: | 1521-3757 0044-8249 |
| DOI: | 10.1002/ange.201813748 |
| Popis: | Photoacoustic (PA) imaging shows promise in the sensitive detection of caspase-3 activated in early tumor apoptosis in response to chemotherapy; smart PA probes are thus in high demand. Herein, we report the first smart PA probe (1-RGD) responsive to caspase-3, enabling real-time and high-resolution imaging of tumor apoptosis. 1-RGD is designed to leverage the synergetic effect of active delivery and caspase-3 activation. It is selectively recognized by active caspase-3 to trigger peptide substrate cleavage and biocompatible macrocyclization-mediated self-assembly, leading to an amplified PA imaging signal and prolonged retention in apoptotic tumor cells. Strong, high-resolution PA images are obtained in chemotherapy-induced apoptotic tumors in living mice after intravenous administration with 1-RGD, facilitating sensitive reporting of caspase-3 activity and distribution within tumor tissues. |
| Databáze: | OpenAIRE |
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