Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues
| Autor: | Arthur M. Friedlander, Jared M. Crumpler, Melissa G. Hunter, Christopher P. Klimko, David E. Harbourt, Christopher K. Cote, Avery V. Quirk, Steven A. Tobery, Jennifer Chua, Jennifer L. Shoe, Donald J. Chabot, Christopher L. Jensen, Joel A. Bozue, Sara I. Ruiz |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Burkholderia pseudomallei Time Factors Yersinia pestis Epidemiology Guinea Pigs 030231 tropical medicine lcsh:Medicine Virulence Burkholderia mallei lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Formaldehyde Animals lcsh:RC109-216 030212 general & internal medicine Francisella tularensis Spores Bacterial Microbial Viability Bacteria biology Research lcsh:R biology.organism_classification Bacillus anthracis Infectious Diseases chemistry Glutaral Organ Specificity Tier 1 Glutaraldehyde Disinfectants |
| Zdroj: | Emerging Infectious Diseases, Vol 25, Iss 5, Pp 919-926 (2019) |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | For safety, designated Select Agents in tissues must be inactivated and viability tested before the tissue undergoes further processing and analysis. In response to the shipping of samples of “inactivated” Bacillus anthracis that inadvertently contained live spores to nonregulated entities and partners worldwide, the Federal Register now mandates in-house validation of inactivation procedures and standardization of viability testing to detect live organisms in samples containing Select Agents that have undergone an inactivation process. We tested and validated formaldehyde and glutaraldehyde inactivation procedures for animal tissues infected with virulent B. anthracis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis. We confirmed that our fixation procedures for tissues containing these Tier 1 Select Agents resulted in complete inactivation and that our validated viability testing methods do not interfere with detection of live organisms. Institutions may use this work as a guide to develop and conduct their own testing to comply with the policy. |
| Databáze: | OpenAIRE |
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