Molecular isolation and sequence determination of the cDNA for the mouse sperm-specific lactate dehydrogenase-X gene
| Autor: | Kun C. Wu, Yun-Fai Lau, Chi-Yu G. Lee, Kaimin Chan |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Biophysics Biology Biochemistry Mice Rapid amplification of cDNA ends Complementary DNA Testis Animals Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene Peptide sequence Southern blot Base Composition Base Sequence L-Lactate Dehydrogenase cDNA library Structural gene Cell Biology DNA Molecular biology Isoenzymes genomic DNA Genes |
| Zdroj: | Biochemical and biophysical research communications. 146(3) |
| ISSN: | 0006-291X |
| Popis: | Several cDNA clones for the mouse lactate dehydrogenase-X (LDH-X), a sperm-specific glycolytic enzyme, were isolated from mouse testicular cDNA libraries constructed in the bacteriophage vectors, lambda gt11 and gt10. The largest cDNA clone contains an insert of 1135 base pairs in length and an open reading frame that encodes a 332 amino acid polypeptide with a molecular weight of 35.89 kD.. The deduced amino acid sequence of this protein is in close agreement with the published sequence of mouse LDH-X obtained by direct protein sequencing. Northern analysis of RNA isolated from different tissues detected a single size mRNA of 1.5 kilobases in mouse testis but not in brain or liver. The Ldh-x structural gene was estimated to be about 12 kb in size as demonstrated by Southern hybridization analysis of mouse genomic DNA using the full-length cDNA as a probe. |
| Databáze: | OpenAIRE |
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