TNF-α modulates the differentiation induced by butyrate in the HT-29 human colon adenocarcinoma cell line
Autor: | Jirina Hofmanova, Alois Kozubík, Z Zadák, Martina Kovaříková, Jiří Pacherník |
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Rok vydání: | 2000 |
Předmět: |
Cancer Research
Apoptosis Butyrate Biology Flow cytometry chemistry.chemical_compound medicine Humans medicine.diagnostic_test Tumor Necrosis Factor-alpha Cell growth Sodium butyrate Cell cycle Alkaline Phosphatase Cadherins Molecular biology Neoplasm Proteins Butyrates Cell Transformation Neoplastic Oncology Biochemistry chemistry Cell culture Alkaline phosphatase HT29 Cells |
Zdroj: | European Journal of Cancer. 36:1844-1852 |
ISSN: | 0959-8049 |
Popis: | The aim of this study was to determine whether and how tumour necrosis factor alpha (TNF-alpha) modulates butyrate effects. After the treatment of human colon adenocarcinoma HT-29 cells with sodium butyrate (NaBt), TNF-alpha or with their combinations we detected cell cycle (flow cytometry), cell proliferation (amidoblack and MTT assays), the amount of dead (floating) and apoptotic cells (flow cytometry and fluorescence microscopy), and the level of differentiation by alkaline phosphatase (ALP) activity (spectrophotometry), relative F-actin content (confocal laser scanning microscopy analysis) and E-cadherin expression (Western blot analysis). Both TNF-alpha and NaBt decreased cell growth in a dose-dependent manner. After combined treatment of the cells with both agents used, either none or additive effects were observed as compared with NaBt treatment alone. The level of dead and apoptotic cells was dose-dependently increased after this combined treatment. In contrast, TNF-alpha suppressed ALP activity and F-actin accumulation induced by NaBt. The results suggest that TNF-alpha does not influence significantly the antiproliferative effects of NaBt but, contrary to its potentiation of apoptosis, it markedly reduces NaBt-induced differentiation of HT-29 colon adenocarcinoma cells. |
Databáze: | OpenAIRE |
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