Isolation, Identification and Quantitation of Testosterone in the Urine of Normal Adults and in Patients with Endocrine Disorders
| Autor: | Claude J. Migeon, Alvro M. Camacho |
|---|---|
| Rok vydání: | 1963 |
| Předmět: |
medicine.medical_specialty
Carcinoma Hepatocellular Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Adrenal Gland Diseases Urine Biochemistry Steroid chemistry.chemical_compound Endocrinology Internal medicine medicine Humans Testosterone Congenital adrenal hyperplasia Androstenedione Chromatography Chemistry Liver Neoplasms Biochemistry (medical) medicine.disease Virilism Paper chromatography Reagent Chromic acid Female |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 23:301-305 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | A procedure for the isolation of testosterone from human urine is presented. Following β-glucuronidase hydrolysis, urine was extracted with ether. The extract was chromatographed on a florisil column prior to gradient elution alumina chromatography and paper chromatography in 2 systems (Bush 1 and Bush 3). The isolated steroid has a mobility in both paper chromatographic systems similar to that of authentic testosterone. This material yielded a bright yellow fluorescent spot when subjected to the alkali-fluorescence test and a blue color with the Zimmermann reagent. Eluates from paper exhibited a UV absorption maximum at 240 mμ. The sulfuric acid and infrared absorption spectra were similar to those of authentic testosterone. The product resulting from chromic acid oxidation of the isolated steroid ran in the Bush 3 system at the same rate as authentic androstenedione. Testosterone has been isolated from the urine of normal adults, patients with untreated congenital adrenal hyperplasia, one of whom was bi... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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