Non-surgical alternatives to invasive procedures in mice
| Autor: | Brendan Lee, P J Garlick, J C Marini |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Blood Specimen Collection medicine.medical_specialty Nitrogen Isotopes General Veterinary business.industry Catheterization Specific Pathogen-Free Organisms Surgery Mice Recovery period Catheter medicine.anatomical_structure Animals Laboratory Anesthesia Urea kinetics Animals Urea Medicine Animal Science and Zoology Sampling (medicine) business Vein Blood sampling |
| Zdroj: | Laboratory Animals. 40:275-281 |
| ISSN: | 1758-1117 0023-6772 |
| DOI: | 10.1258/002367706777611479 |
| Popis: | We have developed and validated catheterization protocols in mice that allow for simultaneous infusion and sampling. A sampling catheter was inserted in the lateral vein of the tail, while the animals were infused either intravenously or intragastrically through a second catheter placed in the contralateral lateral vein or via an intragastric catheter, respectively. The applicability of these methods of infusion and blood sampling were validated by conducting urea kinetics utilizing stable isotopes. These non-surgical procedures are non-invasive, inexpensive, fast to perform and animals do not require a recovery period before their use. |
| Databáze: | OpenAIRE |
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