Prolonged alcohol intake leads to irreversible loss of vasopressin and oxytocin neurons in the paraventricular nucleus of the hypothalamus
| Autor: | Carlos Ruela, Manuel M. Paula-Barbosa, M. Dulce Madeira, Susana M. Silva |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Vasopressin medicine.medical_specialty Alcohol Drinking Vasopressins Gene Expression Neuropeptide Biology Oxytocin Supraoptic nucleus Internal medicine medicine Animals RNA Messenger Rats Wistar Molecular Biology In Situ Hybridization Neurons Ethanol Suprachiasmatic nucleus General Neuroscience Central Nervous System Depressants Organ Size Immunohistochemistry Rats Substance Withdrawal Syndrome Alcoholism medicine.anatomical_structure Endocrinology nervous system Hypothalamus Nerve Degeneration Magnocellular cell Neurology (clinical) Neuron hormones hormone substitutes and hormone antagonists Paraventricular Hypothalamic Nucleus Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 925:76-88 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(01)03261-9 |
| Popis: | Previous data revealed that numerous neurons in the supraoptic nucleus degenerate after prolonged ethanol exposure, and that the surviving neurons increase their activity in order to prevent dramatic changes in water metabolism. Conversely, excess alcohol does not induce cell death in the suprachiasmatic nucleus, but leads to depression of neuropeptide synthesis that is further aggravated by withdrawal. The aim of the present study is to characterize the effects of prolonged ethanol exposure on the magnocellular neurons of the paraventricular nucleus (PVN) in order to establish whether or not magnocellular neurons display a common pattern of reaction to excess alcohol, irrespective of the hypothalamic cell group they belong. Using conventional histological techniques, immunohistochemistry and in situ hybridization, the structural organization and the synthesis and expression of vasopressin (VP) and oxytocin (OXT) in the magnocellular component of the PVN were studied under normal conditions and following chronic ethanol treatment (6 or 10 months) and withdrawal (4 months after 6 months of alcohol intake). After ethanol treatment, there was a marked decrease in the number of VP- and OXT-immunoreactive magnocellular neurons that was attributable to cell death. The surviving neurons were hypertrophied and the VP and OXT mRNA levels in the PVN unchanged. Withdrawal did not alter the number of VP- and OXT-producing neurons or the gene expression of these peptides. These results substantiate the view that after prolonged ethanol exposure numerous neurons of the hypothalamic magnocellular system degenerate, but the mRNA levels of VP and OXT are not decreased due to compensatory changes undergone by the surviving neurons. |
| Databáze: | OpenAIRE |
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