Looking for phosphate-accumulating bacteria in activated sludge processes: a multidisciplinary approach
| Autor: | Bernard Taminiau, Alison Brognaux, Frank Delvigne, Philippe Compère, Julien Bauwens, Georges Daube, Frédéric Francis, Cédric Tarayre, Georges Lognay, Michael Dermience, Evi Michels, Raphaëlle Charlier, Anissa Delepierre, Erik Meers |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Health Toxicology and Mutagenesis Microorganism 030106 microbiology chemistry.chemical_element Biology DNA Ribosomal Waste Disposal Fluid Microbiology Phosphates 03 medical and health sciences chemistry.chemical_compound Environmental Chemistry Food science Bacteria Sewage Phosphorus General Medicine Phosphate Pollution Microscopy Electron 030104 developmental biology Activated sludge Enhanced biological phosphorus removal Wastewater chemistry Sewage treatment Sludge Water Pollutants Chemical |
| Zdroj: | Environmental science and pollution research international. 24(9) |
| ISSN: | 1614-7499 |
| Popis: | Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: α-Proteobacteria, β-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink