The responses of the growth, cytochrome P450 isoenzymes activities and the metabolomics in earthworms to sublethal doses of dichlorvos in soil
| Autor: | Zhang Xuemei, Gong Jiuping, Wei Zhang, Xiu-ying Li, Yang Xiaoxia, Liu Jianfei, Li Dianyan, Junjie Lin, Chai Yong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
CYP2C9
Growth inhibition rate CYP3A4 Health Toxicology and Mutagenesis CYP1A2 0211 other engineering and technologies Phenylalanine 02 engineering and technology 010501 environmental sciences 01 natural sciences Environmental pollution chemistry.chemical_compound Soil Cytochrome P-450 Enzyme System Cytochrome P-450 CYP1A2 Dichlorvos Animals Cytochrome P-450 CYP3A Metabolomics Soil Pollutants GE1-350 Oligochaeta 0105 earth and related environmental sciences chemistry.chemical_classification 021110 strategic defence & security studies Public Health Environmental and Occupational Health General Medicine Metabolism Ornithine Pollution Amino acid Glutamine Isoenzymes Environmental sciences chemistry Biochemistry TD172-193.5 Important metabolites Leucine Biomarkers |
| Zdroj: | Ecotoxicology and Environmental Safety, Vol 207, Iss, Pp 111547-(2021) |
| ISSN: | 0147-6513 |
| Popis: | In this paper, earthworms (Eisenia fetida) were exposed to sublethal doses of dichlorvos (spiked concentration of 0.1, 1.0, 10 mg/kg) in soil for 14 days, the metabolomics and activities of cytochrome P450 (CYP) isoenzymes (CYP1A2, CYP2C9 and CYP3A4) of earthworms were analyzed aiming to identify sensitive biomarkers and reveal possible mode of toxic action. The results showed that CYP1A2 and CYP2C9 activity appeared to be more sensitive than CYP3A4 activity in response to dichlorvos, and that metabolic responses based on the metabolomics depended on both of the length of exposure and exposure dose. Malate, ornithine, glucose, inosine, myo-inositol and some amino acids (glutamine, tryptophan, phenylalanine, tyrosine, leucine, histidine, glutamate, lysine) and CYP isozenzymes may be biomarkers to reveal the toxic effect of dichlorvos on earthworms. Compared to controls, when dichlorvos dose reached 1.0 and 10 mg/kg on day 14, glucose and ornithine increased significantly, malate and some amino acids (glutamine, tryptophan, phenylalanine, tyrosine, leucine) decreased significantly, and activities of CYP1A2 and CYP2C9 were inhibited significantly. The current results suggested that 1.0 and 10 mg/kg dichlorvos for 14 days of exposure blocked energy metabolism, disordered Krebs cycle, interfered amino acids metabolism and evoked toxic effects on earthworms. |
| Databáze: | OpenAIRE |
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