Infectivity of in vitro transcripts of Johnsongrass mosaic potyvirus full-length cDNA clones in maize and sorghum
| Autor: | E. Nurhayati, K.-S. Kim, H.-Y. Oh, K. H. Gough, C. K. Pallaghy, D. D. Shukla, Suranto Suranto |
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| Rok vydání: | 2003 |
| Předmět: |
Infectivity
DNA Complementary biology Strain (chemistry) Base Sequence Transcription Genetic Potyviridae Molecular Sequence Data Potyvirus General Medicine biology.organism_classification Johnsongrass mosaic virus Poaceae Virology Zea mays Virus Protein sequencing Complementary DNA Cloning Molecular Edible Grain Plant Diseases |
| Zdroj: | Archives of virology. 148(3) |
| ISSN: | 0304-8608 |
| Popis: | In vitro transcripts of full-length cDNA clones of the Johnsongrass strain of Johnsongrass mosaic potyvirus (JGMV-Jg) were infectious on maize and sorghum when inoculated by mechanical or by biolistic bombardment. Two of the cDNA clones with spontaneous mutations in the coat protein were not infectious. Sequence differences between infectious and non-infectious transcripts revealed that alteration of inferred amino sequences, near or in the N-terminus of the coat protein, profoundly affected the infectivity of transcripts. Transcripts of chimeric full-length cDNA of JGMV-Jg, containing coat protein sequences from the Krish-infecting strain of JGMV, were infectious in Krish resistant sorghums. |
| Databáze: | OpenAIRE |
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