Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock
| Autor: | Antonio Peña, Ayixon Sánchez-Reyes, Norma Silvia Sánchez, María del Rayo Sánchez-Carbente, Francisco Padilla-Garfias, Jesús Aguirre, Olivia Sánchez, Martha Calahorra, José Raunel Tinoco-Valencia, Yordanis Pérez-Llano, Jorge Luis Folch-Mallol, Nilda del C. Sánchez, Ramón Alberto Batista-García, María Del Rocío Rodríguez-Hernández, Eya Caridad Rodríguez-Pupo |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
osmolyte Osmotic shock QH301-705.5 Chemistry Plant Science Trehalose Halophile chemistry.chemical_compound Aspergillus HOG Biochemistry Osmolyte Arabitol Extremophile Aspergillus sydowii Biology (General) osmotic shock halophile extremophile Cell wall modification Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Fungi Volume 7 Issue 6 Journal of Fungi, Vol 7, Iss 414, p 414 (2021) |
| ISSN: | 2309-608X |
| Popis: | Aspergillus sydowii is a moderate halophile fungus extensively studied for its biotechnological potential and halophile responses, which has also been reported as a coral reef pathogen. In a recent publication, the transcriptomic analysis of this fungus, when growing on wheat straw, showed that genes related to cell wall modification and cation transporters were upregulated under hypersaline conditions but not under 0.5 M NaCl, the optimal salinity for growth in this strain. This led us to study osmolyte accumulation as a mechanism to withstand moderate salinity. In this work, we show that A. sydowii accumulates trehalose, arabitol, mannitol, and glycerol with different temporal dynamics, which depend on whether the fungus is exposed to hypo- or hyperosmotic stress. The transcripts coding for enzymes responsible for polyalcohol synthesis were regulated in a stress-dependent manner. Interestingly, A. sydowii contains three homologs (Hog1, Hog2 and MpkC) of the Hog1 MAPK, the master regulator of hyperosmotic stress response in S. cerevisiae and other fungi. We show a differential regulation of these MAPKs under different salinity conditions, including sustained basal Hog1/Hog2 phosphorylation levels in the absence of NaCl or in the presence of 2.0 M NaCl, in contrast to what is observed in S. cerevisiae. These findings indicate that halophilic fungi such as A. sydowii utilize different osmoadaptation mechanisms to hypersaline conditions. |
| Databáze: | OpenAIRE |
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