Evaluation of a real-time impedance analysis platform on fungal infection
| Autor: | Wenying Cai, Dan Ning, Jiufeng Sun, De Wu, Huiqiong Zhou, Dawei Guan, Huan Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Cell Microbiology Aspergillus fumigatus Mice 03 medical and health sciences Ascomycota Candida albicans Electric Impedance medicine Animals Molecular Biology Cell Proliferation biology Strain (chemistry) Cell growth Fungi biology.organism_classification In vitro Corpus albicans RAW 264.7 Cells medicine.anatomical_structure Mycoses Cell culture |
| Zdroj: | Journal of Microbiological Methods. 136:88-93 |
| ISSN: | 0167-7012 |
| Popis: | End-point assays of in vitro cell proliferation and death have been employed to study the mechanisms of fungal pathogenesis and have shown the responses of host cells at individual time points. A new cell analysis technology has been developed that allows for the continuous measurement and quantification of cell activities, thus enabling the dynamic assessment of electrical impedance when various pathogens are cultured in vitro. In this study, this system was evaluated to determine the response of the cell line RAW264.7 to infection by several clinically relevant fungi in vitro, including Aspergillus fumigatus, Candida albicans, and melanized and albino mutant strains of Fonsecaea monophora. The results showed that infection resulted in rounding of the host cells with a loss of contact between individual cells and a decline in the electrical impedance of all test groups. However, changes in the electrical impedance were variable. Aspergillus fumigatus caused initial increases and later significant decreases in the electrical impedance, while for C. albicans and F. monophora, the effect was reduced. The melanized strain of F. monophora caused a faster change in the electrical impedance than the albino strain. Our data proved that this system can be used as an efficient tool for monitoring cellular responses to fungal infection. |
| Databáze: | OpenAIRE |
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