Identifying long-term memory B-cells in vaccinated children despite waning antibody levels specific for Bordetella pertussis proteins
| Autor: | Reinier H. Veenhoven, Kemal Öztürk, Lotte H. Hendrikx, Lia G. H. de Rond, Elisabeth A. M. Sanders, Anne-Marie Buisman, Guy A. M. Berbers |
|---|---|
| Přispěvatelé: | University of Groningen |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Bordetella pertussis
Whooping Cough SERUM ANTIBODIES MEDIATED-IMMUNITY Immunoglobulin G Memory B-cells INFECTION PROTECTION Child Children Haemophilus Vaccines SEROLOGICAL MEMORY B-Lymphocytes biology Tetanus Antibodies Bacterial Vaccination Infectious Diseases Child Preschool Molecular Medicine VIRUS Pertactin BOOSTER IMMUNIZATION Immunization Secondary Pertussis toxin complex mixtures Pertussis medicine Humans Hepatitis B Vaccines Whooping cough Diphtheria-Tetanus-Pertussis Vaccine General Veterinary General Immunology and Microbiology business.industry Public Health Environmental and Occupational Health ADULTS biology.organism_classification medicine.disease Virology Immunization Immunology Antibody Formation biology.protein business Immunologic Memory WHOLE-CELL RESPONSES |
| Zdroj: | Vaccine, 29(7), 1431-1437. ELSEVIER SCI LTD |
| ISSN: | 0264-410X |
| Popis: | Whooping cough is a respiratory disease caused by Bordetella pertussis. Since the 1950s in developed countries pertussis vaccinations are included in the national immunization program. However, antibody levels rapidly wane after both whole cell and acellular pertussis vaccination. Therefore protection against pertussis may depend largely on long-term B- and T-cell immunities. We investigated long-term pertussis-specific memory B-cell responses in children who were primed at infant age with the Dutch wP-vaccine (ISRCTN65428640). Purified B-cells were characterized by FACS-analysis and after polyclonal stimulation memory B-cells were detected by ELISPOT-assays specific for pertussis toxin, filamentous haemagglutinin, pertactin and tetanus. In addition, plasma IgG levels directed to the same antigens were measured by a fluorescent bead-based multiplex immunoassay. Two and 3 years after wP priming as well as 2 and 5 years after the aP booster at the age of 4, low plasma IgG levels to the pertussis proteins were found. At the same time, however pertussis protein-specific memory B-cells could be detected and their number increased with age. The number of tetanus-specific memory B-cells was similar in all age groups, whereas IgG-tetanus levels were high 2 years after tetanus booster compared to pre- and 5 years post-booster levels. This study shows the presence of long-term pertussis protein-specific memory B-cells in children despite waning antibody levels after vaccination, which suggests that memory B-cells in addition to antibodies may contribute to protection against pertussis. (C) 2010 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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