Mitogen-activated protein kinase kinase 6 is involved in the immune response to bacterial di-/tripeptide challenge in grass carp Ctenopharyngodon idella
| Autor: | Peng Song, Shuangqing Lu, Gao Qianting, Liu Tianting, Ding Xiong, Jinting Liao, Bei Chen, Fufa Qu, Wenjie Luo, Jianzhou Tang, Zhen Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine Carps MAP Kinase Kinase 6 Aquatic Science Mitogen-activated protein kinase kinase Random Allocation 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Complementary DNA Animals Humans Environmental Chemistry Protein kinase A Innate immune system biology Kinase Dipeptides 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Grass carp Cell biology HEK293 Cells 030104 developmental biology chemistry 040102 fisheries 0401 agriculture forestry and fisheries Signal transduction Oligopeptides Muramyl dipeptide |
| Zdroj: | Fish & Shellfish Immunology. 84:795-801 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2018.10.073 |
| Popis: | Mitogen-activated protein kinase kinase 6 (MKK6) is an essential component of the p38MAPK signaling pathway, which is involved in the modulation of inflammation, cell apoptosis and survival responses in mammals. However, the function of MKK6s in teleosts is still unclear. In this study, a fish MKK6 homolog (CiMKK6) was first identified from the grass carp (Ctenopharyngodon idella), a freshwater fish. CiMKK6 cDNA encodes a putative protein of 357 amino acids that contains conserved structural characteristics of the MKK6 family, including the S_TKc domain, SVAKT motif and DVD site. The deduced CiMKK6 protein exhibits high sequence homology with other reported fish MKK6s and shares the closest relationship with MKK6 from Danio rerio. Quantitative real-time PCR (qRT-PCR) analysis revealed that CiMKK6 mRNA was widely expressed in all tested tissues and stages of embryonic development. Additionally, the transcript levels of CiMKK6 in the intestine were significantly upregulated in response to bacterial muramyl dipeptide (MDP) and L-Ala-γ-D-Glu-meso-diaminopimelic acid (Tri-DAP) stimulation. Moreover, subcellular localization analysis indicated that CiMKK6 was distributed in both the cytoplasm and the nucleus of HEK293T cells. Finally, overexpression of CiMKK6 significantly enhanced the transcriptional activity of the AP-1 reporter gene in HEK293T cells. Overall, these findings may help better clarify the immune function of teleost MKK6s and provide new insight into the immune defense mechanisms of grass carp. |
| Databáze: | OpenAIRE |
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