Glucose and leucine uptake by rat dorsal root ganglia is not insulin sensitive
| Autor: | N. J. Patel, J. G. Llewelyn, D. W. Wright, P. K. Thomas |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment ATPase 3-O-Methylglucose In Vitro Techniques Biology Ouabain Rats Sprague-Dawley Leucine Ganglia Spinal Internal medicine Diabetes mellitus medicine Animals Insulin chemistry.chemical_classification Methylglucosides Metabolism medicine.disease Rats Amino acid Glucose Endocrinology Neurology chemistry biology.protein Neurology (clinical) medicine.drug |
| Zdroj: | Journal of the Neurological Sciences. 121:159-162 |
| ISSN: | 0022-510X |
| DOI: | 10.1016/0022-510x(94)90345-x |
| Popis: | Dorsal root ganglia from rats were incubated with 3-O-methyl-[14C]glucose, and [3H]leucine in the presence or absence of insulin in order to determine whether insulin influences the uptake of glucose and amino acids by the cells of the ganglion. No effect was detected. A significant proportion (38%) of the uptake of [3H]leucine was shown to be inhibited by ouabain and therefore energy dependent, utilizing Na+K(+)-ATPase. The activity of this enzyme is known to be impaired in dorsal root ganglia in diabetic rats, as is the uptake of amino acids; these phenomena are therefore unlikely to be due to a direct effect of reduced circulating insulin levels. The relevance of these findings to theories as to the causation of diabetic neuropathy is discussed. |
| Databáze: | OpenAIRE |
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