Comparative genomics of white and opaque cell states supports an epigenetic mechanism of phenotypic switching in Candida albicans
| Autor: | Richard J. Bennett, Iuliana V. Ene, Chapman N. Beekman, Christina A. Cuomo |
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| Přispěvatelé: | Brown University, Broad Institute [Cambridge], Harvard University [Cambridge]-Massachusetts Institute of Technology (MIT), This work was supported by NIH NIAID R21AI139592 and NIH NIGMS IDeA award (P20GM109035) to I.V.E., and by NIH NIAID R01 AI081704 and R01 AI141893 to R.J.B. C.A.C. was supported by NIH NIAID award U19AI110818 to the Broad Institute. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
AcademicSubjects/SCI00010 [SDV]Life Sciences [q-bio] Phenotypic switching comparative genomics AcademicSubjects/SCI01180 MESH: Phenotype Epigenesis Genetic Fungal Proteins 03 medical and health sciences Gene Expression Regulation Fungal Genetic variation Gene expression Candida albicans Genetics Gene Regulatory Networks Epigenetics MESH: Epigenesis Genetic Molecular Biology Genetics (clinical) 030304 developmental biology MESH: Gene Regulatory Networks Comparative genomics 0303 health sciences epigenetic switch biology 030306 microbiology MESH: Candida albicans MESH: Genomics Genomics biology.organism_classification Corpus albicans Genome Report White (mutation) Phenotype AcademicSubjects/SCI00960 MESH: Fungal Proteins MESH: Gene Expression Regulation Fungal |
| Zdroj: | G3 G3, Genetics Society of America, 2021, 11 (2), pp.jkab001. ⟨10.1093/g3journal/jkab001⟩ G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | Several Candida species can undergo a heritable and reversible transition from a ‘white’ state to a mating proficient ‘opaque’ state. This ability relies on highly interconnected transcriptional networks that control cell-type-specific gene expression programs over multiple generations. Candida albicans, the most prominent pathogenic Candida species, provides a well-studied paradigm for the white-opaque transition. In this species, a network of at least eight transcriptional regulators controls the balance between white and opaque states that have distinct morphologies, transcriptional profiles, and physiological properties. Given the reversible nature and the high frequency of white-opaque transitions, it is widely assumed that this switch is governed by epigenetic mechanisms that occur independently of any changes in DNA sequence. However, a direct genomic comparison between white and opaque cells has yet to be performed. Here, we present a whole-genome comparative analysis of C. albicans white and opaque cells. This analysis revealed rare genetic changes between cell states, none of which are linked to white-opaque switching. This result is consistent with epigenetic mechanisms controlling cell state differentiation in C. albicans and provides direct evidence against a role for genetic variation in mediating the switch. |
| Databáze: | OpenAIRE |
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