Optical Configuration Analysis of Hydroxy Fatty Acids in Bacterial Lipids by Chiral Column High-Performance Liquid Chromatography
| Autor: | Yoshifumi Kodani, Tohey Matsuyama, Yoji Nakagawa, Kenichi Kishida |
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| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Elution Fatty Acids Immunology Fatty acid biology.organism_classification Lipids Microbiology Isocyanate High-performance liquid chromatography chemistry.chemical_compound Column chromatography chemistry Biochemistry Virology Pseudomonas aeruginosa Serratia marcescens Composition (visual arts) Derivatization Chromatography High Pressure Liquid |
| Zdroj: | Microbiology and Immunology. 41:27-32 |
| ISSN: | 0385-5600 |
| Popis: | Through the adoption of a chiral stationary phase in high-performance liquid chromatography and a simple derivatization method for hydroxy fatty acids, it became easy to separate and identify the optical isomers of 2- and 3-hydroxy fatty acids composing several kinds of microbial lipids. The 2- and 3-hydroxy fatty acids were converted with dinitrophenyl isocyanate to their 3,5-dinitrophenyl urethane derivatives (DU-derivatives), which were analyzable by HPLC using a chiral column. By varying the composition of an eluent, separation of the DU-derivatives of hydroxy fatty acids differing in optical configuration, chain length and position of hydroxyl group was achieved. The general elution orders of these DU-derivatives were determined with authentic 2- and 3-hydroxy fatty acids. Small amounts (approximately 300 micrograms) of ornithine-containing lipids isolated from the Serratia marcescens strains were examined by this method to identify 3-hydroxy fatty acids of the lipids as D isomers. |
| Databáze: | OpenAIRE |
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