A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles
| Autor: | Mario F. Feldman, Gabriel E. Wagner, Ruth Prassl, Klaus Zangger, Wael Elhenawy, Thomas Blume, Sandro Roier, Joachim Reidl, Günther Daum, Stefan Schild, Lisa Klug |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Microbiology (medical) Proteome Virulence Heterologous Exosomes medicine.disease_cause Microbiology Haemophilus influenzae Protein targeting medicine Animals Antigens Bacterial Mice Inbred BALB C biology Pathogenic bacteria General Medicine biology.organism_classification Lipids 3. Good health Infectious Diseases Bacterial Vaccines Bacterial outer membrane Biogenesis Bacteria |
| Zdroj: | International Journal of Medical Microbiology. 305:298-309 |
| ISSN: | 1438-4221 |
| DOI: | 10.1016/j.ijmm.2014.12.005 |
| Popis: | Outer membrane vesicles (OMVs) are spherical and bilayered particles that are naturally released from the outer membrane (OM) of Gram-negative bacteria. They have been proposed to possess several biological roles in pathogenesis and interbacterial interactions. Additionally, OMVs have been suggested as potential vaccine candidates against infections caused by pathogenic bacteria like Haemophilus influenzae, a human pathogen of the respiratory tract. Unfortunately, there is still a lack of fundamental knowledge regarding OMV biogenesis, protein sorting into OMVs, OMV size and quantity, as well as OMV composition in H. influenzae. Thus, this study comprehensively characterized and compared OMVs and OMs derived from heterologous encapsulated as well as nonencapsulated H. influenzae strains. Semiquantitative immunoblot analysis revealed that certain OM proteins are enriched or excluded in OMVs suggesting the presence of regulated protein sorting mechanisms into OMVs as well as interconnected OMV biogenesis mechanisms in H. influenzae. Nanoparticle tracking analysis, transmission electron microscopy, as well as protein and lipooligosaccharide quantifications demonstrated that heterologous H. influenzae strains differ in their OMV size and quantity. Lipidomic analyses identified palmitic acid as the most abundant fatty acid, while phosphatidylethanolamine was found to be the most dominant phospholipid present in OMVs and the OM of all strains tested. Proteomic analysis confirmed that H. influenzae OMVs contain vaccine candidate proteins as well as important virulence factors. These findings contribute to the understanding of OMV biogenesis as well as biological roles of OMVs and, in addition, may be important for the future development of OMV based vaccines against H. influenzae infections. |
| Databáze: | OpenAIRE |
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