Evaluation of PCR-based methods for the diagnosis of infection in bancroftian filariasis
| Autor: | Senarath Dissanayake, Joaquim Norões, Gerusa Dreyer, Willy F. Piessens, Abraham Rocha, Zulma Medeiros |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pathology
medicine.medical_specialty Helminthiasis Biology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Microfilaria law.invention Filariasis Serology law parasitic diseases Blood plasma medicine Humans Polymerase chain reaction Public Health Environmental and Occupational Health General Medicine DNA Helminth medicine.disease DNA extraction Infectious Diseases Wuchereria bancrofti Parasitology |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 94:526-530 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/s0035-9203(00)90075-0 |
| Popis: | The value of the polymerase chain reaction (PCR) in the diagnosis of Wuchereria bancrofti infection was evaluated in comparison to microscopical examination of night blood smears, Nuclepore filtration, serology and ultrasonography. No correlation was found between PCR-based deoxyribonucleic acid (DNA) probing and serology. We did not find any evidence of free filarial DNA in either blood plasma or chylocoele fluid. We conclude that the 2 PCR-based techniques evaluated are not more sensitive than Nuclepore filtration for detection of W. bancrofti microfilaraemia, need at least 1 intact microfilaria in the volume of blood used for DNA extraction, and were much inferior to ultrasonography for detection of amicrofilaraemic adult worm carriers. |
| Databáze: | OpenAIRE |
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