CYCLOPS, a mediator of symbiotic intracellular accommodation
| Autor: | Yoshikatsu Murooka, Kate Vickers, Jillian Perry, Makoto Hayashi, Catharine White, Judith M. Müller, Shusei Sato, Mari Banba, Trevor L. Wang, Katharina Markmann, Bettina Schuller, Haruko Imaizumi-Anraku, Satoshi Tabata, Sylvia Singh, Satoko Yoshida, Erika Asamizu, Martin Parniske, Masayoshi Kawaguchi, Koji Yano |
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| Rok vydání: | 2008 |
| Předmět: |
Rhizobiaceae
Positional cloning Molecular Sequence Data Nuclear Localization Signals Mutant Lotus japonicus Organogenesis Cyclops Rhizobia Bimolecular fluorescence complementation Nutrigenomics Nitrogen Fixation Botany Calcium Signaling Glomeromycota Symbiosis Plant Diseases Plant Proteins Multidisciplinary biology fungi Intracellular Signaling Peptides and Proteins food and beverages Oryza Fabaceae Biological Sciences biology.organism_classification Cell biology Mutation Lotus Commentary Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 105:20540-20545 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The initiation of intracellular infection of legume roots by symbiotic rhizobia bacteria and arbuscular mycorrhiza (AM) fungi is preceded by the induction of calcium signatures in and around the nucleus of root epidermal cells. Although a calcium and calmodulin-dependent kinase (CCaMK) is a key mediator of symbiotic root responses, the decoding of the calcium signal and the molecular events downstream are only poorly understood. Here, we characterize Lotus japonicus cyclops mutants on which microbial infection was severely inhibited. In contrast, nodule organogenesis was initiated in response to rhizobia, but arrested prematurely. This arrest was overcome when a deregulated CCaMK mutant version was introduced into cyclops mutants, conferring the development of full-sized, spontaneous nodules. Because cyclops mutants block symbiotic infection but are competent for nodule development, they reveal a bifurcation of signal transduction downstream of CCaMK. We identified CYCLOPS by positional cloning. CYCLOPS carries a functional nuclear localization signal and a predicted coiled-coil domain. We observed colocalization and physical interaction between CCaMK and CYCLOPS in plant and yeast cell nuclei in the absence of symbiotic stimulation. Importantly, CYCLOPS is a phosphorylation substrate of CCaMK in vitro. Cyclops mutants of rice were impaired in AM, and rice CYCLOPS could restore symbiosis in Lotus cyclops mutants, indicating a functional conservation across angiosperms. Our results suggest that CYCLOPS forms an ancient, preassembled signal transduction complex with CCaMK that is specifically required for infection, whereas organogenesis likely requires additional yet-to-be identified CCaMK interactors or substrates. |
| Databáze: | OpenAIRE |
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