Non-digestible oligosaccharides modulate intestinal immune activation and suppress cow's milk allergic symptoms
| Autor: | Kerperien, J, Jeurink, P V, Wehkamp, T, van der Veer, A, van de Kant, H J G, Hofman, G A, van Esch, E C A M, Garssen, J, Willemsen, L E M, Knippels, L M J, Sub General Pharmacology, Sub Immunopharmacology, Sub Developmental Biology, Pharmacology |
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| Přispěvatelé: | Sub General Pharmacology, Sub Immunopharmacology, Sub Developmental Biology, Pharmacology |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Immunology Oligosaccharides Milk allergy Complex Mixtures regulatory T cells Immunomodulation Mice Intestinal mucosa prevention T-Lymphocyte Subsets Transforming Growth Factor beta Food allergy Internal medicine medicine cow’s milk allergy Animals Humans Immunology and Allergy Sensitization Mice Inbred C3H biology business.industry non-digestible oligosaccharides FOXP3 food and beverages Forkhead Transcription Factors Allergens medicine.disease Immunity Innate Small intestine Interleukin-10 Milk medicine.anatomical_structure Endocrinology intestinal mucosa Dietary Supplements Pediatrics Perinatology and Child Health biology.protein Cattle Digestion Immunization Milk Hypersensitivity Antibody business Anaphylaxis |
| Zdroj: | Pediatric Allergy and Immunology, 25(8), 747. Blackwell Munksgaard |
| ISSN: | 0905-6157 |
| Popis: | BACKGROUND: Cow's milk allergy is a common food allergy in childhood and no effective preventive or curative treatment is available. This study aimed at comparing single short-chain galacto- (scGOS), long-chain fructo- (lcFOS) or pectin-derived acidic oligosaccharides (pAOS) and/or mixtures of scGOS/lcFOS (GF) or scGOS/lcFOS/pAOS (GFA) to prevent or treat food allergy. METHODS: In the preventive protocol, C3H/HeOuJ mice were fed diets containing single oligosaccharides or mixtures GF or GFA throughout the study protocol. In the treatment protocol, GF or GFA was provided for 4 wk starting after the last sensitization. The allergic skin response and anaphylaxis scores were determined, after oral challenge whey-specific immunoglobulins were measured, and qPCR for T-cell markers and Foxp3 counts using immunohistochemistry were performed on the small intestine and colon. RESULTS: Only in the preventive setting, the GF or GFA mixture, but not the single oligosaccharides, reduced the allergic skin response and whey-IgG1 levels in whey-sensitized mice, compared to the control diet. Both GF and GFA increased the number of Foxp3+ cells in the proximal small intestine of whey - compared to sham-sensitized mice. Expression of Th2 and Th17 mRNA markers increased in the middle part of the small intestine of whey-sensitized mice, which was prevented by GF. By contrast, GFA enhanced Tbet (Th1), IL-10 and TGF-β mRNA expression compared to GF which was maintained in the distal small intestine and/or colon. CONCLUSIONS: Dietary supplementation with scGOS/lcFOS or scGOS/lcFOS/pAOS during sensitization, both effectively reduce allergic symptoms but differentially affect mucosal immune activation in whey-sensitized mice. |
| Databáze: | OpenAIRE |
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