Impaired Fibrinolysis and Traumatic Brain Injury in Mice
| Autor: | Tracy K. McIntosh, David I. Graham, Valeria Conte, Diego M. Morales, Scott Fujimoto, M. Sean Grady, Sherman C. Stein |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Genetically modified mouse medicine.medical_specialty Plasmin Traumatic brain injury Ratón medicine.medical_treatment Morris water navigation task Mice Transgenic Neuroprotection Functional Laterality Mice Cognition Internal medicine Fibrinolysis medicine Animals Thromboplastin Maze Learning Mice Knockout business.industry medicine.disease Immunohistochemistry Urokinase-Type Plasminogen Activator Endocrinology Brain Injuries Immunology Female Neurology (clinical) Intracranial Thrombosis business medicine.drug |
| Zdroj: | Journal of Neurotrauma. 23:976-984 |
| ISSN: | 1557-9042 0897-7151 |
| DOI: | 10.1089/neu.2006.23.976 |
| Popis: | Traumatic brain injury (TBI) has been associated with intravascular coagulation, which may be a result of thromboplastin released following brain injury. Clots thus formed are lysed by plasmin, which is activated by tissue-type and urokinase-type plasminogen activators (uPA). To evaluate the association between traumatic intravascular coagulation and post-traumatic outcome, uPA knockout (uPA-/-) transgenic mice (n=12) or wild-type littermates (WT; n=12) were anesthetized and subjected to controlled cortical impact (CCI) brain injury. A second group of uPA-/- (n=12) and WT mice (n=12) were subjected to sham injury. Motor function was assessed over 2 weeks using the composite neuroscore test and cognition (learning) was assessed with the Morris Water Maze (MWM) at 2 weeks post-injury, whereupon the animals were sacrificed for cortical lesion volume analysis. Motor function was significantly worse in the brain-injured uPA-/- mice when compared to brain-injured WT mice at 48 h (p |
| Databáze: | OpenAIRE |
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