Tight Skin 2 Mice Exhibit Delayed Wound Healing Caused by Increased Elastic Fibers in Fibrotic Skin
| Autor: | Chelsea M. Burgwin, Elizabeth P. Blankenhorn, Kristen B. Long, Richard B. Huneke, Carol M. Artlett |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Delayed wound healing
Pathology medicine.medical_specialty medicine.diagnostic_test Anti-nuclear antibody integumentary system business.industry Wound size Critical Care and Intensive Care Medicine medicine.disease Extracellular matrix Fibrosis Biopsy Emergency Medicine medicine Tight skin Wound healing business Discovery Express |
| Popis: | Rationale: The Tight Skin 2 (Tsk2) mouse model of systemic sclerosis (SSc) has many features of human disease, including tight skin, excessive collagen deposition, alterations in the extracellular matrix (ECM), increased elastic fibers, and occurrence of antinuclear antibodies with age. A tight skin phenotype is observed by 2 weeks of age, but measurable skin fibrosis is only apparent at 10 weeks. We completed a series of wound healing experiments to determine how fibrosis affects wound healing in Tsk2/+ mice compared with their wild-type (WT) littermates. Method: We performed these experiments by introducing four 4 mm biopsy punched wounds on the back of each mouse, ventral of the midline, and observed wound healing over 10 days. Tsk2/+ mice showed significantly delayed wound healing and increased wound size compared with the WT littermates at both 5 and 10 weeks of age. We explored the potential sources of this response by wounding Tsk2/+ mice that were genetically deficient either for the NLRP3 inflamm... |
| Databáze: | OpenAIRE |
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