Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis
| Autor: | Bruna Mara Aparecida de Carvalho, Gleidson Giordano Pinto de Carvalho, Junio Cota, Sérgio Henrique Sousa Santos, Igor Viana Brandi, Jocilane Pereira de Oliveira, Ederson Paulo Xavier Guilherme, Lorendane Millena de Carvalho |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Immobilized enzyme Aspergillus oryzae Clinical Biochemistry 01 natural sciences Biochemistry Analytical Chemistry Fungal Proteins chemistry.chemical_compound Bioreactors 010608 biotechnology Bioreactor Amylase Maltose Chromatography biology Hydrolysis 010401 analytical chemistry Temperature food and beverages Starch Hydrogen-Ion Concentration biology.organism_classification Enzymes Immobilized 0104 chemical sciences Starch hydrolysis Monomer chemistry Acrylamide biology.protein alpha-Amylases Porosity Cryogels |
| Zdroj: | Electrophoresis. 38(22-23) |
| ISSN: | 1522-2683 |
| Popis: | A bioreactor was built by means of immobilizing alpha-amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44mg amylase/gcriogel, concentration of starch solution 45 g/L and temperature of 35°C. The immobilized enzyme showed a conversion ratio ranging from 68.2 to 97.37%, depending on the pH and temperature employed. Thus, our results suggest that the alpha-amylase from A. oryzae immobilized on cryogel monoliths represents a potential process for industrial production of maltose from starch hydrolysis. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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