The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
| Autor: | Satoru Moritoh, Namie Murayama, Reiko Daigaku, Kota Sato, Kazuko Omodaka, Hiroshi Tawarayama, Yukihiro Shiga, Takeshi Yabana, Koji M. Nishiguchi, Kazuichi Maruyama, Shigeto Maekawa, Kosuke Fujita, Toru Nakazawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Retinal Ganglion Cells N-Methylaspartate genetic structures Science Excitotoxicity Inflammation Apoptosis Pharmacology medicine.disease_cause Neuroprotection Retinal ganglion Retina Article 03 medical and health sciences Hesperidin chemistry.chemical_compound Mice 0302 clinical medicine Retinal Diseases medicine Animals Cells Cultured Multidisciplinary biology Chemistry Calpain Tumor Necrosis Factor-alpha eye diseases Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Neuroprotective Agents Treatment Outcome Biochemistry biology.protein Medicine Evoked Potentials Visual sense organs medicine.symptom 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | We found that hesperidin, a plant-derived bioflavonoid, may be a candidate agent for neuroprotective treatment in the retina, after screening 41 materials for anti-oxidative properties in a primary retinal cell culture under oxidative stress. We found that the intravitreal injection of hesperidin in mice prevented reductions in markers of the retinal ganglion cells (RGCs) and RGC death after N-methyl-D-aspartate (NMDA)-induced excitotoxicity. Hesperidin treatment also reduced calpain activation, reactive oxygen species generation and TNF-α gene expression. Finally, hesperidin treatment improved electrophysiological function, measured with visual evoked potential, and visual function, measured with optomotry. Thus, we found that hesperidin suppressed a number of cytotoxic factors associated with NMDA-induced cell death signaling, such as oxidative stress, over-activation of calpain, and inflammation, thereby protecting the RGCs in mice. Therefore, hesperidin may have potential as a therapeutic supplement for protecting the retina against the damage associated with excitotoxic injury, such as occurs in glaucoma and diabetic retinopathy. |
| Databáze: | OpenAIRE |
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