Use of two different methods for the electron microscope radioautographic detection of labeled antibodies bound to thin sections
| Autor: | B M Kopriwa, J M Paiement |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Histology
Materials science Thyroid Gland Thyroglobulin Antibodies law.invention Iodine Radioisotopes Colloid symbols.namesake chemistry.chemical_compound law Animals Histocytochemistry Vesicle Endoplasmic reticulum Radiochemistry Resolution (electron density) Golgi apparatus Rats Microscopy Electron chemistry Emulsion symbols Autoradiography Glutaraldehyde Anatomy Electron microscope |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 26(10) |
| ISSN: | 0022-1554 |
| Popis: | Thyroglobulin antigemcity has been located by exposure to labeled antibodies of thin sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate. Two methods were used for the handling of sections during antibody exposure and emulsion coating. In the first method, sections were labeled while floating freely within plastic “0” rings over solutions of ‘251-labeled antithyroglobulin antibodies and were then mounted on celloidin coated slides to be processed for electron microscope raclioautography by the dipping technique. In the second method, sections were first mounted on electron microscope grids that were then floated over the ‘251-labeled antibody solution; the sections were then coated with emulsion by means of a wire loop. Qualitative and quantitative comparison of the results obtained with the two methods shows that they basically give the same results, e.g., they disclose the same pattern of specific labeling within the follicular colloid, colloid droplets, apical vesicles, Golgi and rough endoplasmic reticulum. A slightly lower half distance value for the second method (340 A) as compared to the first (460 A) indicates that the second method may provide a slightly better resolution, presumably because only with the second method, the labeled section surface is in immediate contact with the emulsion. It may be concluded, however, that both methods provide a reasonably satisfactory detection of radioactive antibodies. |
| Databáze: | OpenAIRE |
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