A medium-throughput screen for inhibitors of human metapneumovirus
| Autor: | Jennifer Becker, John V. Williams, David Weaver, Sharon J. Tollefson |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
viruses Cell Drug Evaluation Preclinical Microbial Sensitivity Tests Biology Virus Replication Antiviral Agents Virus lcsh:Infectious and parasitic diseases statins Cell Line Cell membrane 03 medical and health sciences paramyxovirus Human metapneumovirus Drug Discovery medicine Animals Humans lcsh:RC109-216 Serial Passage high-throughput screen Lower respiratory infection Respiratory Tract Infections 030304 developmental biology 0303 health sciences Budding topoisomerase inhibitors 030306 microbiology General Medicine biology.organism_classification Virology 3. Good health medicine.anatomical_structure Viral replication Original Article Metapneumovirus Topoisomerase inhibitor |
| Zdroj: | Antiviral Chemistry and Chemotherapy Antiviral Chemistry & Chemotherapy Antiviral Chemistry & Chemotherapy, Vol 27 (2019) |
| ISSN: | 2040-2066 |
| Popis: | Human metapneumovirus, a paramyxovirus discovered in 2001, is a major cause of lower respiratory infection in adults and children worldwide. There are no licensed vaccines or drugs for human metapneumovirus. We developed a fluorescent, cell-based medium-throughput screening assay for human metapneumovirus that captures inhibitors of all stages of the viral lifecycle except budding of progeny virus particles from the cell membrane. We optimized and validated the assay and performed a successful medium-throughput screening. A number of hits were identified, several of which were confirmed to inhibit viral replication in secondary assays. This assay offers potential to discover new antivirals for human metapneumovirus and related respiratory viruses. Compounds discovered using the medium-throughput screening may also provide useful probes of viral biology. |
| Databáze: | OpenAIRE |
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