The Antibacterial Assay of Tectorigenin with Detergents or ATPase Inhibitors against Methicillin-ResistantStaphylococcus aureus
Autor: | Dong-Yeul Kwon, Ok-Hwa Kang, Kuang-Shim Lee, Myong-Soo Chong, Dong-Sung Lee, Jang-Gi Choi, Sung-Bae Kim, Dae-Ki Joung, Su-Hyun Mun, Youn-Chul Kim, Dong-Won Shin, Ryong Gong |
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Rok vydání: | 2014 |
Předmět: |
Tectorigenin
endocrine system Article Subject biology Membrane permeability business.industry ATPase nutritional and metabolic diseases lcsh:Other systems of medicine lcsh:RZ201-999 medicine.disease_cause Antimicrobial Microbiology Multiple drug resistance chemistry.chemical_compound Complementary and alternative medicine chemistry Staphylococcus aureus medicine biology.protein Peptidoglycan business Antibacterial activity Research Article |
Zdroj: | Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2014 (2014) |
ISSN: | 1741-4288 1741-427X |
DOI: | 10.1155/2014/716509 |
Popis: | Tectorigenin (TTR) is an O-methylated isoflavone derived from the rhizome ofBelamacanda chinensis(L.) DC. It is known to perform a wide spectrum of biological activities such as antioxidant, anti-inflammatory, anti-tumor. The aim of this study is to examine the mechanism of antibacterial activity of TTR against methicillin-resistantStaphylococcus aureus(MRSA). The anti-MRSA activity of TTR was analyzed in combination assays with detergent, ATPase inhibitors, and peptidoglycan (PGN) derived fromS. aureus. Transmission electron microscopy (TEM) was used to monitor survival characteristics and changes inS. aureusmorphology. The MIC values of TTR against all the tested strains were 125 μg/mL. The OD(600) of each suspension treated with a combination of Triton X-100, DCCD, and NaN3with TTR (1/10 × MIC) had been reduced from 68% to 80%, compared to the TTR alone. At a concentration of 125 μg/mL, PGN blocked antibacterial activity of TTR. This study indicates that anti-MRSA action of TTR is closely related to cytoplasmic membrane permeability and ABC transporter, and PGN at 125 μg/mL directly bind to and inhibit TTR at 62.5 μg/mL. These results can be important indication in study on antimicrobial activity mechanism against multidrug resistant strains. |
Databáze: | OpenAIRE |
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