Bovine cumulus-oocyte-complex-quality is reflected in sensitivity for alpha-amanitin, oocyte-diameter and developmental capacity

Autor: A.A.C. de Wit, Th.A.M. Kruip
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Male
endocrine system
medicine.medical_specialty
Amanitins
Radiata
Cattle reproductive technology
Fertilization in Vitro
Biology
Andrology
Embryonic and Fetal Development
Endocrinology
Germinal vesicle breakdown
Food Animals
Oxazines
medicine
Animals
Microscopy
Phase-Contrast
Blastocyst
Zona pellucida
Coloring Agents
Instituut voor Dierhouderij en Diergezondheid
Zona Pellucida
Amanitin
Nucleic Acid Synthesis Inhibitors
Gynecology
Germinal vesicle
Staining and Labeling
ID-Lelystad
urogenital system
Embryo
General Medicine
Oocyte
biology.organism_classification
ID Lelystad
medicine.anatomical_structure
ID-Lelystad
Instituut voor Dierhouderij en Diergezondheid
ID Lelystad
Institute for Animal Science and Health
Oocytes
Regression Analysis
α-Amanitin
Animal Science and Zoology
Cattle
Female
Follicle Stimulating Hormone
In vitro embryo production
Institute for Animal Science and Health
Zdroj: Animal Reproduction Science 65 (2001)
Animal Reproduction Science, 65, 51-65
ISSN: 0378-4320
DOI: 10.1016/s0378-4320(00)00215-3
Popis: The aim of the present study was to find more parameters to define developmental competence of cumulus-oocyte-complexes (COCs). Bovine COCs were divided into five groups based on their morphology. In order of increasing level of atresia: COC-A had a bright, compact cumulus investment; COC-B1 also had a compact cumulus investment, but was darker than COC-A; the color of COC-B2 was comparable with COC-B1 but the corona radiata appeared to dissociate from the rest of the cumulus investment; the cumulus of COC-B3 was almost black and the corona radiata was almost completely dissociated from the rest of the cumulus investment; COC-C had a strongly expanded cumulus investment with dark spots of degenerated cells. An increasing level of atresia was accompanied by: (1) an increasing zona pellucida diameter (147.6, 150.8, 151.0, 154.3 and 155.1 microm, respectively, for COC-A, COC-B1, COC-B2, COC-B3 and COC-C); (2) an increasing oocyte diameter except for COC-C (120.9, 122.8, 122.8, 123.9 and 118.4 microm, respectively); (3) an increasing developmental competence except for COC-C (13.9, 14.7, 17.4, 19.1 and 11.5%, respectively, development to morula and blastocyst after in vitro embryo production (IVP)) and (4) by a increasing percentage of oocytes exhibiting germinal vesicle breakdown (GVBD) after 24h culture with alpha-amanitin and FSH, except for COC-A (34.4, 68.6, 51.0, 22.0 and 4.8%, respectively, oocytes arrested in GV). In general, embryo-quality, expressed in nuclei-count, was significantly affected by COC-quality (P0.05) with B3B1B2AC. However, if the developmental stages were compared separately, this effect was less evident. It was found that, in absence of FSH, alpha-amanitin was unable to inhibit GVBD and that the success of GVBD inhibition was positively correlated to the amount of cumulus cells surrounding the oocyte. COCs that had been exposed to alpha-amanitin and FSH during maturation retained the ability to cleave after IVF, but were unable to develop any further.
Databáze: OpenAIRE
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