Vinegar-processed Curcuma phaeocaulis promotes anti-angiogenic activity and reduces toxicity in zebrafish and rat models

Autor: Lei Wang, Zongping Zhu, Yenchit Techadamrongsin, Rui Li, Jia-Li Yu, Tianhui Gao, Boonjai Limsila, Wan Liao, Jiao Chen, Chaomei Fu, Yi Chen
Rok vydání: 2021
Předmět:
Male
Rat model
cardiotoxicity
Pharmaceutical Science
Angiogenesis Inhibitors
Decoction
ezhu
RM1-950
Blood stasis
Pharmacology
Raw material
ovarian coefficient
030226 pharmacology & pharmacy
01 natural sciences
Animals
Genetically Modified
Rats
Sprague-Dawley
uterine coefficient
03 medical and health sciences
Curcuma
0302 clinical medicine
Drug Discovery
Oils
Volatile
Animals
Zebrafish
Acetic Acid
Cardiotoxicity
Dose-Response Relationship
Drug
Neovascularization
Pathologic
biology
Plant Extracts
intersegmental blood vessels
Chemistry
General Medicine
biology.organism_classification
Rats
lc50 values
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Complementary and alternative medicine
Toxicity
Molecular Medicine
Female
Zingiberaceae
Therapeutics. Pharmacology
volatile oil
Research Article
Zdroj: Pharmaceutical Biology, Vol 59, Iss 1, Pp 410-417 (2021)
Pharmaceutical Biology
article-version (VoR) Version of Record
ISSN: 1744-5116
1388-0209
DOI: 10.1080/13880209.2021.1874427
Popis: Context Processing with vinegar could enhance the efficacy and reduce the toxicity of Curcuma phaeocaulis Valeton. (Zingiberaceae), a Chinese herbal medicine with anti-inflammatory and antitumor activities. Objective This study investigated the vinegar processing effects by evaluating anti-angiogenic effect and toxicity of C. phaeocaulis through zebrafish and rat models. Materials and methods Zebrafish embryos (AB and FLk-GFP strain) were applied to evaluate toxicity, cardiotoxicity and anti-angiogenic activity of volatile oil, and water decoction of the raw and vinegar-processed C. phaeocaulis. Meanwhile, a blood stasis syndrome rat model was applied to study the toxicity by measuring the ovarian and uterine coefficient. Results Curcuma phaeocaulis volatile oil and its vinegar-processed products in zebrafish had an LC50 of 67.315 and 95.755 μg/mL, respectively. Curcuma phaeocaulis water decoction and its vinegar-processed products had an LC50 of 161.440 and 206.239 μg/mL, respectively. The toxicity of vinegar-processed products was significantly lower than the raw, and the development characteristic of zebrafish embryos at different times confirmed these results. The volatile oil of vinegar-processed products could inhibit the growth of intersegmental blood vessels at the dose of 20 μg/mL, while the raw materials did not exhibit such effect at the same concentration. The rat experiment also confirmed that the volatile oil could reduce toxicity of ovarian and uterine. Discussion and conclusions The study indicated that processing using vinegar could decrease toxicity and increase anti-angiogenic activity of C. phaeocaulis, which could be applied for clinical treatment. Further in-depth study on the synergism and detoxification mechanism of vinegar processing technology is needed.
Databáze: OpenAIRE
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