Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2–related factor 2–antioxidant response element signaling pathway in human endothelial cells
| Autor: | Takahiro Ogawa, Naoaki Morihara, Kei Hiramatsu, Tadamitsu Tsuneyoshi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
NF-E2-Related Factor 2 Glutamate-Cysteine Ligase Endocrinology Diabetes and Metabolism Active Transport Cell Nucleus Biology medicine.disease_cause Plant Roots Antioxidants Umbilical vein 03 medical and health sciences 0302 clinical medicine Endocrinology Gene expression Human Umbilical Vein Endothelial Cells medicine Humans Antioxidant Response Elements RNA Small Interfering Garlic Cells Cultured Cell Nucleus Nutrition and Dietetics Plant Extracts GCLM Molecular biology KEAP1 Heme oxygenase Oxidative Stress 030104 developmental biology Enzyme Induction 030220 oncology & carcinogenesis Dietary Supplements RNA Interference Endothelium Vascular Signal transduction Heme Oxygenase-1 Oxidative stress Signal Transduction |
| Zdroj: | Nutrition Research. 36:143-149 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/j.nutres.2015.09.018 |
| Popis: | The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway defends cells against oxidative stress and regulates the cellular redox balance. Activation of this pathway induces a variety of antioxidant enzymes, resulting in the protection of our bodies against oxidative damage. It has been reported that aged garlic extract (AGE), a garlic preparation that is rich in water-soluble cysteinyl moieties, reduces oxidative stress and helps to ameliorate of cardiovascular, renal and hepatic diseases. We hypothesized that AGE enhances the expression of antioxidant enzymes via the Nrf2-ARE pathway in human umbilical vein endothelial cells in culture. Gene expression of antioxidant enzymes was measured using real-time polymerase chain reaction. Nuclear accumulation of Nrf2 and antioxidant enzymes expression were evaluated using western blotting analyses. We found that AGE promoted the accumulation of Nrf2 into the nucleus in a time- and dose-dependent manner and increased the gene expression and polypeptide level of heme oxygenase-1 (HO-1) and glutamate-cysteine ligase modifier subunit (GCLM). Moreover, the effect of AGE in elevating the gene expression of HO-1 and GCLM was found to be mediated via Nrf2 activation in human umbilical vein endothelial cells. Taken together, these observations suggest that AGE induces the expression of HO-1 and GCLM, which are antioxidant enzymes, via activation of the Nrf2-ARE signaling pathway. |
| Databáze: | OpenAIRE |
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