Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures
| Autor: | Christian Wagner, Rick Dannert, Claudius Moritz Lehr, Siham Hachi, E. Lucumi Moreno, Ronan M. T. Fleming, Khalid I. W. Kane, Roland Sanctuary, Jörg Baller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Matrigel Materials science Microfluidics General Physics and Astronomy lcsh:QC1-999 Shear rate 03 medical and health sciences 030104 developmental biology Particle image velocimetry Cell culture Self-healing hydrogels Fluorescence microscope Seeding lcsh:Physics Biomedical engineering |
| Zdroj: | AIP Advances, Vol 8, Iss 12, Pp 125332-125332-11 (2018) |
| ISSN: | 2158-3226 |
| Popis: | Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem cell-derived cell lines. The use of Matrigel as a surrogate extracellular matrix in microfluidic systems is challenging due to its biochemical, biophysical, and biomechanical properties. Therefore, understanding and characterising these properties is a prerequisite for optimal use of Matrigel in microfluidic systems. We used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device. Using fluorescence microscopy and fluorescent beads for particle image velocimetry measurements (velocity profiles) in combination with classical rheological measurements of Matrigel (viscosity versus shear rate), we characterised the shear rates experienced by cells in a microfluidic device for three-dimensional cell culture. This study provides a better understanding of the mechanical stress experienced by cells, during seeding of a mixture of hydrogel and cells, into three-dimensional microfluidic cell culture devices. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|