Neurotoxins Distinguish Between Different Neuronal Nicotinic Acetylcholine Receptor Subunit Combinations
| Autor: | Steve Heinemann, Jim Patrick, Scott W. Rogers, Kuniro Tsuji, Charles W. Luetje, Stewart N. Abramson, Keiji Wada |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Macromolecular Substances
Protein subunit Neurotoxins Xenopus Gene Expression Mollusk Venoms Alpha (ethology) Receptors Nicotinic Biology Transfection Peptides Cyclic Biochemistry Beta-1 adrenergic receptor Xenopus laevis Cellular and Molecular Neuroscience Cnidarian Venoms medicine Animals Neurotoxin Beta (finance) Neurons Terpenes Bungarotoxins biology.organism_classification Nicotinic acetylcholine receptor nervous system Oocytes Biophysics Female Conotoxins Neuroscience Acetylcholine medicine.drug |
| Zdroj: | Journal of Neurochemistry. 55:632-640 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1990.tb04180.x |
| Popis: | Neuronal and muscle nicotinic acetylcholine receptor subunit combinations expressed in Xenopus oocytes were tested for sensitivity to various neurotoxins. Extensive blockade of the alpha 3 beta 2 neuronal subunit combination was achieved by 10 nM neuronal bungarotoxin. Partial blockade of the alpha 4 beta 2 neuronal and alpha 1 beta 1 gamma delta muscle subunit combinations was caused by 1,000 nM neuronal bungarotoxin. The alpha 2 beta 2 neuronal subunit combination was insensitive to 1,000 nM neuronal bungarotoxin. Nearly complete blockade of all neuronal subunit combinations resulted from incubation with 2 nM neosurugatoxin, whereas 200 nM neosurugatoxin was required for partial blockade of the alpha 1 beta 1 gamma delta muscle subunit combination. The alpha 2 beta 2 and alpha 3 beta 2 neuronal subunit combinations were partially blocked by 10,000 nM lophotoxin analog-1, whereas complete blockade of the alpha 4 beta 2 neuronal and alpha 1 beta 1 gamma delta muscle subunit combinations resulted from incubation with this concentration of lophotoxin analog-1. The alpha 1 beta 1 gamma delta muscle subunit combination was blocked by the alpha-conotoxins G1A and M1 at concentrations of 100 nM. All of the neuronal subunit combinations were insensitive to 10,000 nM of both alpha-conotoxins. Thus, neosurugatoxin and the alpha-conotoxins distinguish between muscle and neuronal subunit combinations, whereas neuronal bungarotoxin and lophotoxin analog-1 distinguish between different neuronal subunit combinations on the basis of differing alpha subunits. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text