Reuteran and levan as carbohydrate sinks in transgenic sugarcane
| Autor: | Iban Eduardo, Jan P. I. Bekker, Johann M. Rohwer, R. Bauer, Jens Kossmann, Lafras Uys, Johannes H. van Wyk, Carin E. Basson |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Sucrose
Starch Plant Science Biology Polysaccharide Tissue Culture Techniques chemistry.chemical_compound Fructan Bacterial Proteins Polysaccharides Genetics Biomass Carbon Radioisotopes Transgenes Glucans Glucan chemistry.chemical_classification Glycosyltransferases Fructose Carbohydrate Plants Genetically Modified Carbon Fructans Saccharum Lactobacillus chemistry Biochemistry Callus |
| Zdroj: | Planta. 236(6) |
| ISSN: | 1432-2048 |
| Popis: | The present study reports the effect of high molecular weight bacterial fructan (levan) and glucan (reuteran) on growth and carbohydrate partitioning in transgenic sugarcane plants. These biopolymers are products of bacterial glycosyltransferases, enzymes that catalyze the polymerization of glucose or fructose residues from sucrose. Constructs, targeted to different subcellular compartments (cell wall and cytosol) and driven by the Cauliflower mosaic virus-35S: maize-ubiquitin promoter, were introduced into sugarcane by biolistic transformation. Polysaccharide accumulation severely affected growth of callus suspension cultures. Regeneration of embryonic callus tissue into plants proved problematic for cell wall-targeted lines. When targeted to the cytosol, only plants with relative low levels of biopolymer accumulation survived. In internodal stalk tissue that accumulate reuteran (max 0.03 mg/g FW), sucrose content (ca 60 mg/g FW) was not affected, while starch content ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink