Radiosensitization of mouse sarcoma cells by fludarabine (F-ara-A) or gemcitabine (dFdC), two nucleoside analogues, is not mediated by an increased induction or a repair inhibition of DNA doublestrand breaks as measured by pulsed-field gel electrophoresis
| Autor: | M Octave Prignot, B M De Coster, Pierre Scalliet, Vincent Grégoire, Marc Beauduin, M Bruniaux |
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| Rok vydání: | 1998 |
| Předmět: |
Radiation-Sensitizing Agents
DNA Repair Fibrosarcoma Antineoplastic Agents Biology Deoxycytidine Radiation Tolerance Ionizing radiation Mice chemistry.chemical_compound Tumor Cells Cultured medicine Animals Radiology Nuclear Medicine and imaging Clonogenic assay Gel electrophoresis Mice Inbred C3H Radiological and Ultrasound Technology DNA Neoplasm Combined Modality Therapy Gemcitabine Molecular biology Electrophoresis Gel Pulsed-Field Fludarabine chemistry Cell culture Immunology Nucleoside Vidarabine DNA DNA Damage medicine.drug |
| Zdroj: | International Journal of Radiation Biology. 73:511-520 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/095530098142059 |
| Popis: | PURPOSE: To investigate the effect of fludarabine (F-ara-A) and gemcitabine (dFdC), two radiosensitizing nucleoside analogues, on the induction and repair of DNA dsb after ionizing radiation. MATERIALS AND METHODS: Radiosensitization of mouse sarcoma SA-NH and FSA cells was studied using a clonogenic assay. Cell survival curves were fitted with the linear-quadratic model. DNA dsbs were detected by pulsed-field gel electrophoresis under neutral conditions. RESULTS: F-ara-A (100 micromol dm(-3) for 1 h prior to irradiation) induced a substantial radiosensitization in SA-NH cells with a dose modification factor of 2.0 for a surviving fraction of 0.5. In a FSA mouse sarcoma cell line, dFdC (5 micromol dm(-3) for 3 h prior to irradiation) induced a modest radiosensitization with a DMF of 1.2 for a surviving fraction of 0.5. Under similar experimental conditions, neither F-ara-A nor dFdC altered the yield of radiation-induced DNA dsbs in the dose range of 0-40 Gy. After a single dose of 25 Gy (SA-NH cells) or 40 Gy (FSA cells), neither the kinetics of repair nor the amount of residual damage was affected by F-ara-A or dFdC. CONCLUSIONS: For experimental conditions under which radiosensitization was observed, neither the induction nor the repair of DNA dsbs after ionizing radiation were affected by F-ara-A or dFdC. |
| Databáze: | OpenAIRE |
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