A Description of the Possible Etiology of the Cilantro Yellow Blotch Disease
| Autor: | Maher Al Rwahnih, Olufemi J. Alabi, Brianna C. Gaytán, Cecilia Villegas |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Coriandrum viruses Plant Science Whitefly Nicotiana occidentalis 01 natural sciences California 03 medical and health sciences Crinivirus Sativum Alfalfa mosaic virus Lettuce chlorosis virus Plant Diseases Chlorosis biology food and beverages biology.organism_classification Texas Horticulture 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Disease. 104:630-633 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-09-19-1958-sc |
| Popis: | A virus-like disease characterized by foliar yellow blotch symptoms and resembling those described for cilantro yellow blotch disease in California was observed in a 4.05-ha cilantro (Coriandrum sativum) cv. Santo field in Hidalgo County, Texas during spring 2019. Disease incidence at harvest was estimated at ∼20%, and the affected plants were rendered unmarketable. Foliar systemic chlorosis symptoms were observed on sap-inoculated Nicotiana occidentalis plants (n = 3) using inocula from symptomatic cilantro. Total RNA aliquots from 11 randomly collected leaf tissue samples (symptomatic = 7, asymptomatic = 4) were pooled into a composite cilantro RNA sample which was analyzed by high throughput sequencing (HTS). Analyses of the obtained 15.7 million raw reads (76 nt each) yielded virus-specific contigs that mapped to the genomes of alfalfa mosaic virus (AMV), beet pseudoyellows virus (BPYV), and lettuce chlorosis virus (LCV). Virus-specific primers designed from the HTS-derived sequences were used to screen the samples in two-step RT-PCR assays, resulting in the detection of AMV+BPYV in 3 of 7 symptomatic cilantro samples, AMV+LCV in 4 of 7 symptomatic cilantro samples, and AMV alone in the 4 asymptomatic cilantro and sap-inoculated N. occidentalis samples. The results represent the first reports of the natural infection of cilantro by BPYV and LCV and implicate the mixed infection of a Crinivirus and AMV in cilantro yellow blotch disease. |
| Databáze: | OpenAIRE |
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