Fatigue-induced Fos immunoreactivity within the lumbar cord and amygdala decreases after С60 fullerene pretreatment
| Autor: | Agnieszka Borowik, Inna V. Sokolowska, Jacek Piosik, Olena P. Mankivska, Volodymyr O. Maisky, Bulgakova Nv, A. V. Maznychenko, Alexander I. Kostyukov, Kamila Butowska, Olga O. Gonchar, Tomasz Tomiak |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Multidisciplinary Chemistry Central nucleus of the amygdala lcsh:R Central nervous system lcsh:Medicine Stimulation Spinal cord Amygdala 03 medical and health sciences Electrophysiology 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Endocrinology Nociception Triceps surae muscle Internal medicine medicine lcsh:Q lcsh:Science 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-67034-1 |
| Popis: | The fundamental aspects related to the mechanisms of action of C60 fullerene nanoparticles on the level of the central nervous system in different experimental conditions are still unclear. Electrophysiological investigation and immunohistochemical techniques of c-fos expression were combined to determine which neural elements within the lumbar segments and in the central nucleus of the amygdala (CeA) are activated under skeletal muscle fatigue development with prior application of C60 fullerenes (dissolved in dimethyl sulfoxide and in distilled water, FDS). After high-frequency electrical stimulation of the triceps surae muscle, the main fatigue-related increases in the c-Fos expression level were registered ipsilaterally within lamina 1 and 5 of the lumbar segments and within the contralateral capsular part of the CeA. C60 fullerene pretreatment in animals with subsequent electrical stimulation induced a distinct (2–4 times) decrease in the level of Fos immunoreactivity in the observed structures in comparison with only fatigue-induced rats. It can be supposed that FDS, as antioxidant compound, can decrease the concentration of free radicals in fatigued tissue and reduce the transmission intensity of nociceptive information from muscles to the spinal cord and amygdala, thereby changing the level of c-Fos expression within the lumbar segments and CeA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|