Production of a Monoclonal Antibody Directed against the High-Affinity Nerve Growth Factor Receptor
| Autor: | C. Ghirelli, Luciano Lombardi, Sylvie Ménard, Laura Asnaghi, Piera Aiello, Fabio Castiglioni, M. G. Borrello, Elda Tagliabue, C. Longhi |
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| Rok vydání: | 1999 |
| Předmět: |
0301 basic medicine
Cancer Research Nerve growth factor IB Clinical Biochemistry Receptors Nerve Growth Factor Biology Proto-Oncogene Mas Tropomyosin receptor kinase C Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine Growth factor receptor Proto-Oncogene Proteins Tumor Cells Cultured Animals Humans Low-affinity nerve growth factor receptor Growth factor receptor inhibitor Receptor trkA Microscopy Immunoelectron Insulin-like growth factor 1 receptor Mice Inbred BALB C Fibroblast growth factor receptor 2 Antibodies Monoclonal Receptor Protein-Tyrosine Kinases 3T3 Cells Fibroblast growth factor receptor 4 Molecular biology 030104 developmental biology nervous system Oncology 030220 oncology & carcinogenesis |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1724-6008 |
| DOI: | 10.1177/172460089901400203 |
| Popis: | The high-affinity nerve growth factor receptor corresponds to the tyrosine protein kinase encoded by the proto-oncogene trkA. Different findings suggest that nerve growth factor (NGF) can be operative in the growth modulation of tumor cell lines possessing high-affinity binding sites for this molecule. Using as immunizing material the SKNBE neuroblastoma cell line transfected with proto-trkA we produced a monoclonal antibody (MAb) able to recognize the high-affinity nerve growth factor receptor. The selected MAb, designated MGR12, is directed against an epitope present on the extracellular domain of the receptor since it showed reactivity on living trkA-expressing cells and was able to immunoprecipitate the proto-trkA molecule. The MGR12 MAb is directed against a non-functional epitope since it neither inhibited NGF binding nor induced receptor internalization. This new reagent appears to be an appropriate tool for analyzing the expression of high-affinity nerve growth factor receptor in tumors of different origin and for elucidating its involvement in tumor progression. |
| Databáze: | OpenAIRE |
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