In vivo retinal and choroidal hypoxia imaging using a novel activatable hypoxia-selective near-infrared fluorescent probe
| Autor: | Genichiro Kishino, Tetsuro Oshika, Simone Beheregaray, Toshiharu Yamashita, Masumi Nagano, Masahiro Fukuda, Osamu Ohneda, Hideko Nagasawa, Sujin Hoshi, Itsuki Kawano, Kensuke Okuda, Shinichi Fukuda |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty genetic structures Fundus Oculi Fundus (eye) Biology 010402 general chemistry 01 natural sciences 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Mice 0302 clinical medicine Retinal Diseases In vivo medicine Electroretinography Animals Humans Fluorescein Angiography Hypoxia Cells Cultured Fluorescent Dyes Retinal Vascular Occlusion Retina Spectroscopy Near-Infrared medicine.diagnostic_test Choroid Reproducibility of Results Retinal Retinopathy of prematurity medicine.disease Fluorescein angiography eye diseases Sensory Systems 0104 chemical sciences Mice Inbred C57BL Ophthalmology Disease Models Animal medicine.anatomical_structure Spectrometry Fluorescence chemistry 030221 ophthalmology & optometry sense organs Endothelium Vascular Rabbits Preclinical imaging |
| Zdroj: | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. 254(12) |
| ISSN: | 1435-702X |
| Popis: | Retinal hypoxia plays a crucial role in ocular neovascular diseases, such as diabetic retinopathy, retinopathy of prematurity, and retinal vascular occlusion. Fluorescein angiography is useful for identifying the hypoxia extent by detecting non-perfusion areas or neovascularization, but its ability to detect early stages of hypoxia is limited. Recently, in vivo fluorescent probes for detecting hypoxia have been developed; however, these have not been extensively applied in ophthalmology. We evaluated whether a novel donor-excited photo-induced electron transfer (d-PeT) system based on an activatable hypoxia-selective near-infrared fluorescent (NIRF) probe (GPU-327) responds to both mild and severe hypoxia in various ocular ischemic diseases animal models. The ocular fundus examination offers unique opportunities for direct observation of the retina through the transparent cornea and lens. After injection of GPU-327 in various ocular hypoxic diseases of mouse and rabbit models, NIRF imaging in the ocular fundus can be performed noninvasively and easily by using commercially available fundus cameras. To investigate the safety of GPU-327, electroretinograms were also recorded after GPU-327 and PBS injection. Fluorescence of GPU-327 increased under mild hypoxic conditions in vitro. GPU-327 also yielded excellent signal-to-noise ratio without washing out in vivo experiments. By using near-infrared region, GPU-327 enables imaging of deeper ischemia, such as choroidal circulation. Additionally, from an electroretinogram, GPU-327 did not cause neurotoxicity. GPU-327 identified hypoxic area both in vivo and in vitro. |
| Databáze: | OpenAIRE |
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