Treatment assessment by monitoring parasite load in skin biopsies from patients with cutaneous leishmaniasis, using quantitative nucleic acid sequence-based amplification
| Autor: | Henk D. F. H. Schallig, W. F. van der Meide, H J C de Vries, Jim E. Zeegelaar, P. P. A. M. van Thiel, W.R. Faber, Inge Peekel |
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| Přispěvatelé: | Infectious diseases, KIT: Biomedical Research, Medical Microbiology and Infection Prevention, Amsterdam institute for Infection and Immunity, Amsterdam Public Health, Dermatology |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Treatment outcome Antiprotozoal Agents Leishmaniasis Cutaneous Dermatology Gastroenterology Parasite load Cutaneous leishmaniasis Internal medicine Biopsy Treatment assessment medicine Animals Humans Prospective Studies Stage (cooking) Prospective cohort study Self-Sustained Sequence Replication Aged Skin medicine.diagnostic_test business.industry Leishmaniasis Middle Aged medicine.disease Treatment Outcome Cryotherapy Female business Follow-Up Studies |
| Zdroj: | Clinical and experimental dermatology, 33(4), 394-399. Wiley-Blackwell |
| ISSN: | 1365-2230 0307-6938 |
| DOI: | 10.1111/j.1365-2230.2007.02680.x |
| Popis: | Summary Background. Current diagnostic methods for cutaneous leishmaniasis (CL) have low sensitivity or are not useful for treatment follow-up. We previously described the quantitative nucleic acid sequence-based amplification (QT-NASBA) method as a sensitive and specific assay for detection and quantification of Leishmania parasites in skin biopsies. This assay could be a valuable instrument for monitoring response to treatment of CL and identifying treatment failures at an early stage. Aim. QT-NASBA results of skin biopsies at the end and 6 weeks after treatment from patients with proven CL on various treatment regimens were compared with clinical outcome. Methods. The QT-NASBA assay measured the parasite load in skin biopsies before, at the end and 6 weeks after treatment. The results were compared with treatment outcome (clinical cure, delayed healing response or treatment failure) up to 6 months after treatment. Results. In total, 137 skin biopsies were obtained from 53 patients. A positive QT-NASBA result 6 weeks after treatment was significantly associated with treatment failure/delayed healing up to 6 months (P |
| Databáze: | OpenAIRE |
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