Imaging Intratumoral Convection: Pressure-Dependent Enhancement in Chemotherapeutic Delivery to Solid Tumors
| Autor: | Ian M. Buchanan, Jason A. Koutcher, Matthew W. Motley, William M. Spees, Terence P. Gade, Yousef Mazaheri |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Magnetic Resonance Spectroscopy Bioenergetics Mice Nude Antineoplastic Agents Convection Article Phosphates Mice In vivo Extracellular fluid Pressure medicine Extracellular Animals Humans Collagenases Cytotoxicity medicine.diagnostic_test Chemistry Magnetic resonance spectroscopic imaging Extracellular Fluid Magnetic resonance imaging Magnetic Resonance Imaging Molecular Weight Oncology Collagenase Cancer research Fluorouracil Colorectal Neoplasms Energy Metabolism HT29 Cells medicine.drug |
| Zdroj: | Clinical Cancer Research. 15:247-255 |
| ISSN: | 1557-3265 1078-0432 |
| DOI: | 10.1158/1078-0432.ccr-08-0611 |
| Popis: | Purpose: Low–molecular weight (LMW) chemotherapeutics are believed to reach tumors through diffusion across capillary beds as well as membrane transporters. Unexpectedly, the delivery of these agents seems to be augmented by reductions in tumor interstitial fluid pressure, an effect typically associated with high–molecular weight molecules that reach tumors principally through convection. We investigated the hypothesis that improved intratumoral convection can alter tumor metabolism and enhance the delivery of a LMW chemotherapeutic agent to solid tumors.Experimental Design: For this purpose, we applied 31P/19F magnetic resonance spectroscopy (MRS) and magnetic resonance spectroscopic imaging (MRSI) to examine the influence of type I collagenase on tumor bioenergetics and the delivery of 5-fluorouracil (5FU) to HT29 human colorectal tumors grown s.c. in mice.Results: Collagenase effected a 34% reduction in tumor interstitial fluid pressure with an attendant disintegration of intratumoral collagen. Neither mice-administered collagenase nor controls receiving PBS showed changes in 31phosphorus MRS–measured tumor bioenergetics; however, a time-dependent increase in the content of extracellular inorganic phosphate (Pie) was observed in tumors of collagenase-treated animals. 31Phosphorus MRSI showed that this increase underscored a more homogeneous distribution of Pie in tumors of experimental mice. 19Fluorine MRS showed that these changes were associated with a 50% increase in 5FU uptake in tumors of experimental versus control animals; however, this increase resulted in an increase in 5FU catabolites rather than fluoronucleotide intermediates that are required for subsequent cytotoxicity.Conclusions: These data indicate that the modulation of convective flow within tumors can improve the delivery of (LMW) chemotherapeutics and show the potential role for noninvasive imaging of this process in vivo. |
| Databáze: | OpenAIRE |
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