Alpha-B crystallin in the normal human myocardium and cardiac conducting system
| Autor: | IH Leach, James Lowe, Tsang Ml, Church Rj |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Bundle of His Biology Pathology and Forensic Medicine Fetal Heart Crystallin Heart Conduction System Pregnancy medicine Humans Intermediate filament Cytoskeleton Aged Sinoatrial Node Aged 80 and over Myocardium Cardiac muscle Middle Aged Bundle branches Crystallins Immunohistochemistry eye diseases Cell biology medicine.anatomical_structure Lens (anatomy) Pregnancy Trimester Second cardiovascular system Atrioventricular Node Desmin Female sense organs Immunostaining |
| Zdroj: | The Journal of pathology. 173(3) |
| ISSN: | 0022-3417 |
| Popis: | The alpha crystallins are major protein components of the ocular lens and show both structural and functional homology with the family of small heat shock proteins. alpha B crystallin is also present in various extra-lenticular tissues, with a high concentration in cardiac muscle. In this study, the myocardium and conducting system from 15 adult and 25 fetal and infant hearts were examined by immunohistochemistry using a previously characterized antiserum to alpha B crystallin. Contractile myocardium showed moderate staining, with particular localization to Z bands and intercalated discs. Fibres of the sino-atrial and atrio-ventricular nodes and His bundle showed less intense staining than contractile fibres, whereas fibres of the left and right bundle branches showed more intense staining. This distribution is similar to that previously demonstrated for the intermediate filament desmin. This observation, together with currently available evidence, suggests that cardiac alpha B crystallin may play a role in protecting the cytoskeleton during cell stress. For practical purposes, immunostaining with alpha B crystallin greatly facilitates the identification of cardiac conducting fibres. |
| Databáze: | OpenAIRE |
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