Gene expression profiles of esophageal squamous cell cancers in Hodgkin lymphoma survivors versus sporadic cases

Autor: Ykema, Berbel L. M., Hoefnagel, Sanne J. M., Rigter, Lisanne S., Kodach, Liudmila L., Meijer, Gerrit A., van Leeuwen, Flora E., Khan, Hina N., Snaebjornsson, Petur, Aleman, Berthe M. P., Broeks, Annegien, Meijer, Sybren L., Wang, Kenneth K., Carvalho, Beatriz, Krishnadath, Kausilia K., vanLeerdam, Monique E., Meijer, S. L., Hulshof, M. C. C. M., Geijsen, E. D., van Laarhoven, H. W. M., van Berge Henegouwen, M. I., Gisbertz, S. S.
Přispěvatelé: Pathology, Epidemiology and Data Science, Surgery, VU University medical center, CCA - Cancer biology and immunology, Graduate School, Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology, Radiotherapy, Oncology
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Oncology
Male
Esophageal Neoplasms
Molecular biology
medicine.medical_treatment
Cancer Treatment
Gene Expression
Sequencing techniques
Mathematical and Statistical Techniques
Cancer Survivors
Gene expression
Breast Tumors
Medicine and Health Sciences
Aged
80 and over
Principal Component Analysis
Multidisciplinary
Statistics
RNA sequencing
Middle Aged
Hodgkin Disease
Esophageal Tissue
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Physical Sciences
Carcinoma
Squamous Cell
Medicine
Female
Research Article
Signal Transduction
Clinical Oncology
Adult
medicine.medical_specialty
Science
Radiation Therapy
Young Adult
Downregulation and upregulation
Internal medicine
Breast Cancer
medicine
Genetics
Humans
Squamous Cell Carcinoma
Esophagus
Statistical Methods
Aged
Colorectal Cancer
Squamous cell cancer
Biology and life sciences
business.industry
Carcinoma
RNA
Cancers and Neoplasms
Radiation therapy
Research and analysis methods
Molecular biology techniques
Multivariate Analysis
Hodgkin lymphoma
Clinical Medicine
business
Transcriptome
Mathematics
Zdroj: PLoS ONE
PLoS ONE, 15(12). PUBLIC LIBRARY SCIENCE
PLoS ONE, 15(12 December):e0243178. Public Library of Science
PLoS ONE, Vol 15, Iss 12, p e0243178 (2020)
on behalf of GIOCA-OES¶ 2020, ' Gene expression profiles of esophageal squamous cell cancers in Hodgkin lymphoma survivors versus sporadic cases ', PLoS ONE, vol. 15, no. 12 December, e0243178 . https://doi.org/10.1371/journal.pone.0243178
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0243178
Popis: Hodgkin lymphoma (HL) survivors are at increased risk of developing second primary esophageal squamous cell cancer (ESCC). We aimed to gain insight in the driving events of ESCC in HL survivors (hESCC) by using RNA sequencing and NanoString profiling. Objectives were to investigate differences in RNA signaling between hESCC and sporadic ESCC (sESCC), and to look for early malignant changes in non-neoplastic esophageal tissue of HL survivors (hNN-tissue). We analyzed material of 26 hESCC cases, identified via the Dutch pathology registry (PALGA) and 17 sESCC cases from one academic institute and RNA sequencing data of 44 sESCC cases from TCGA. Gene expression profiles for the NanoString panel PanCancer IO 360 were obtained from 16/26 hESCC and four hNN-tissue, while non-neoplastic squamous tissue of four sporadic cases (sNN-tissue) served as reference profile. Hierarchical clustering, differential expression and pathway analyses were performed. Overall, the molecular profiles of hESCC and sESCC were similar. There was increased immune, HMGB1 and ILK signaling compared to sNN-tissue. The profiles of hNN-tissue were distinct from sNN-tissue, indicating early field effects in the esophagus of HL survivors. The BRCA1 pathway was upregulated in hESCC tissue, compared to hNN tissue. Analysis of expression profiles reveals overlap between hESCC and sESCC, and differences between hESCC and its surrounding hNN-tissue. Further research is required to validate our results and to investigate whether the changes observed in hNN-tissue are already detectable before development of hESCC. In the future, our findings could be used to improve hESCC patient management.
Databáze: OpenAIRE
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