Induction of ornithine decarboxylase in macrophages by bacterial lipopolysaccharides (LPS) and mycobacterial cell wall material
| Autor: | William K. Nichols, Frederick H. Prosser |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Lipopolysaccharides
genetic structures Lipopolysaccharide Carboxy-Lyases medicine.medical_treatment 8-Bromo Cyclic Adenosine Monophosphate Biology Cycloheximide Ornithine Decarboxylase General Biochemistry Genetics and Molecular Biology Cell Line Ornithine decarboxylase Cell wall Mice chemistry.chemical_compound Adjuvants Immunologic Cell Wall Cyclic AMP medicine Animals Macrophage General Pharmacology Toxicology and Pharmaceutics Dose-Response Relationship Drug Cell growth Macrophages Prostaglandins E fungi General Medicine Mycobacterium bovis Molecular biology In vitro chemistry Enzyme Induction Adjuvant |
| Zdroj: | Life Sciences. 27:913-920 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(80)90100-9 |
| Popis: | Lipopolysaccharide from E. Coli (LPS) and BCG cell walls (BCGcw) are recognized immunoadjuvants that directly stimulate some macrophage functions. The macrophage cell line J774.1 and peritoneal exudate cells (PEC) from mice can be stimulated by LPS or other adjuvants in vitro to synthesize and release protein factor(s) that activate thymus-derived lymphocytes. We have utilized J774.1 cells and PEC to demonstrate that an increase in ornithine decarboxylase (ODC) activity is a marker of early biochemical changes in adjuvant-stimulated macrophages. BCGcw and LPS increased ODC within 2 hours in J774.1 cells as well as murine peritoneal exudate macrophages. Maximal increases in ODC were detected 4 hours after the addition of adjuvants to J774.1 cells. The marked increases (12–23 fold) in ODC observed with BCGcw (20 μg/ml) did not appear to involve an effect on cell proliferation which was suppressed by this adjuvant. Cycloheximide inhibited the induction of ODC by LPS and BCGcw in the macrophage cell line. Evidence that the induction of ODC may be promoted by an increase in cyclic AMP was provided by experiments demonstrating that prostaglandin E1 (PGE1) and 8-bromo-adenosine-3′:5′-monophosphate (8Br-cyclic AMP) can mimic the effects of LPS and BCGcw in J774.1 cells. These observations indicate that one of the early biochemical changes in macrophages promoted by adjuvants is an induction of ODC. |
| Databáze: | OpenAIRE |
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