Role of the adenylate cyclase, phosphoinositidase C and receptor tyrosyl kinase systems in the control of hepatocyte proliferation by hepatocyte growth factor
| Autor: | Gwyn W. Gould, A. J. Marker, E. Galloway, S. Palmer, R. N. M. Macsween, Taichi Nakamura, M. Bushfield |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Platelet-derived growth factor Inositol Phosphates Adenylate kinase Biology Biochemistry chemistry.chemical_compound Epidermal growth factor Cyclic AMP medicine Animals Hepatectomy Phosphorylation Cells Cultured Pharmacology Hepatocyte Growth Factor Phosphoric Diester Hydrolases DNA Protein-Tyrosine Kinases Molecular biology Liver Regeneration Rats Enzyme Activation medicine.anatomical_structure Liver chemistry Hepatocyte biology.protein Hepatocyte growth factor Cyclase activity Tyrosine kinase Cell Division Platelet-derived growth factor receptor Adenylyl Cyclases medicine.drug |
| Zdroj: | Biochemical Pharmacology. 44:1037-1043 |
| ISSN: | 0006-2952 |
| Popis: | Hepatocyte growth factor (HGF) is the most potent known mitogen for hepatocytes in primary culture. However, the mechanisms through which HGF induces hepatocyte proliferation have not been defined. Here we have investigated the role of the adenylate cyclase, phosphoinositidase C and tyrosine kinase signalling systems in the control of hepatocyte proliferation by HGF using freshly isolated or cultured adult rat hepatocytes. We show that human recombinant HGF caused a dose-dependent increase in hepatocyte DNA synthesis with a maximal effect at 10 ng/mL and an EC50 of 5.9 ng/mL. HGF had no effect on hepatocyte adenylate cyclase activity or intracellular cAMP levels. Elevation of hepatocyte cAMP levels resulted in inhibition of HGF-stimulated DNA synthesis. HGF stimulated inositol phospholipid hydrolysis with a maximal effect at 25 ng/mL and potentiated the effect of vasopressin (10(-8) and 10(-9)M). HGF (100 ng/mL) caused an increase in the phosphorylation on tyrosine of an unknown hepatocyte protein with a molecular mass of 36 kDa. Thus, we have shown that HGF, like epidermal growth factor (EGF), can activate the phosphoinositidase C and tyrosine kinase systems in rat hepatocytes. As with EGF, these intracellular signalling systems may underlie HGF-induced hepatocyte proliferation. |
| Databáze: | OpenAIRE |
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