Bacteriocin of Enterococcus from lactoserum able to cause oxidative stress in Staphylococcus aureus
| Autor: | Alberto Jorge Eraso, Albesa Inés |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Staphylococcus aureus
Erythrocytes Neutrophils Biophysics Pseudomonas fluorescens medicine.disease_cause Hemolysis Biochemistry Microbiology chemistry.chemical_compound Bacteriocins Bacteriocin Superoxides medicine Humans Molecular Biology Molecular mass biology Superoxide Immune Sera Hemolysin Pigments Biological Cell Biology biology.organism_classification Molecular Weight Oxidative Stress chemistry Luminescent Measurements Oligopeptides Enterococcus Oxidative stress Bacteria |
| Zdroj: | Biochemical and Biophysical Research Communications. 314:897-901 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2003.12.179 |
| Popis: | The effect of a bacteriocin of Enterococcus on the oxidative metabolism of sensitive bacteria was investigated through the detection of oxidative stress by chemiluminescence (CL). The bacteriocin named EntB was purified to study the action on Staphylococcus aureus isolated from cosmetic. Chromatographic separation of EntB indicated different states of oligomerization with molecular weights multiple of 12,000 Da monomeric form. The monomer purified by ion exchange was studied in its capacity to affect the oxidative metabolism of S. aureus , which showed increase of anion superoxide (O 2 − ) when incubated with EntB. This effect was compared to the action of EntB on leukocytes as an assay of toxicity. EntB did not generate significant oxidative stress in leukocytes. Pyoverdin, a leukotoxic pigment of Pseudomonas fluorescens , was taken as reference, and it was found that this pigment caused similar oxidative stress to EntB in S. aureus ; however, pyoverdin generated high production of anion superoxide (O 2 − ) in leukocytes, while EntB did not increase the level of O 2 − . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect